Evaluation of high resolution computed tomography findings of cystic fibrosis

Ayse Senay Sasihuseyinoglu; Derya Ufuk Altıntaş; Sureyya Soyupak; Dilek Dogruel; Mustafa Yılmaz; Mahir Serbes; Gulsah Duyuler

doi:10.3904/kjim.2017.287

Korean J Intern Med > Volume 34(2); 2019 > Article

Sasihuseyinoglu, Altıntaş, Soyupak, Dogruel, Yılmaz, Serbes, and Duyuler: Evaluation of high resolution computed tomography findings of cystic fibrosis

Original Article

Pulmonology

Korean J Intern Med 2019;34(2):335-343.

Published online: July 6, 2018

DOI: https://doi.org/10.3904/kjim.2017.287

Evaluation of high resolution computed tomography findings of cystic fibrosis

Ayse Senay Sasihuseyinoglu¹, Derya Ufuk Altıntaş¹, Sureyya Soyupak², Dilek Dogruel¹, Mustafa Yılmaz¹, Mahir Serbes¹, Gulsah Duyuler¹

¹Department of Pediatric Allergy and Immunology, Cukurova University School of Medicine, Adana, Turkey

²Department of Radiology, Cukurova University School of Medicine, Adana, Turkey

Correspondence to Ayse Senay Sasihuseyinoglu, M.D. Department of Pediatric Allergy and Immunology, Balcali Hospital, Cukurova University School of Medicine, 01330 Balcali, Adana, Turkey Tel: +90-506-608-3040 Fax: +90-322-338-6900 E-mail: ssashuseyinoglu@yahoo.com.tr

Received August 18, 2017 Revised December 6, 2017 Accepted March 13, 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background/Aims

Morphological changes due to lung disease in patients with cystic fibrosis (CF) were evaluated using high resolution computed tomography (HRCT), and the HRCT scores obtained using the Bhalla scoring system were correlated with those obtained using clinical and laboratory indicators.

Methods

Medical records of 28 children with CF who underwent chest CT in Department of Pediatric Allergy and Immunology, Cukurova University Balcali Hospital between March 2011 and January 2016 were retrospectively reviewed. Demographic data and physical examination, respiratory cultures, pulmonary function tests, and chest HRCT findings were evaluated. Patients were divided into the following two groups according to their forced expiratory volume in the first second (FEV₁) values: normal FEV₁ (≥ 80% of predicted values) and low FEV₁ (< 80% of predicted values). Deep throat or sputum cultures were evaluated for the presence of Pseudomonas aeruginosa (PsA) and other bacteria. HRCT scans were scored using the Bhalla scoring system.

Results

No significant correlation was found between the Bhalla scores and sex, age group, or height percentiles. Significant relationships were found between the Bhalla score and weight (p = 0.036) and body mass index (BMI) (p = 0.032) percentiles below the third percentile, bacterial growth in the sputum/ deep throat cultures (p = 0.009), and presence of PsA (p = 0.004). Moreover, a significant correlation was found between the Bhalla score and FEV₁ (r = −0.315, p = 0.0272), forced vital capacity (FVC; r = −0.381, p = 0.0178), forced expiratory flow between 25% and 75% of FVC (r = −0.229, p = 0.0431), and BMI (r = −3.368, p = 0.050).

Conclusions

Chest HRCT is an important diagnostic tool for the pulmonary evaluation of children with CF.

Keywords: Cystic fibrosis; Child; Computed tomography; Pulmonary evaluation

INTRODUCTION

Cystic fibrosis (CF), caused by a mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, is an inherited, chronic, progressive, and fatal disease [1]. Deletion in CFTR leads to dehydration of the airway surface liquid layer, reduction in mucociliary clearance, and chronic bronchopulmonary infection in the lungs [2]. Detection of early CF lung disease is essential for understanding disease progression and providing endpoints for clinical trials. However, this is difficult because of the lack of sensitive noninvasive outcome measures [3]. Pulmonary function tests (PFTs) and chest radiographs have been used to monitor lung structure in patients with CF. However, PFTs require active participation from patients to perform the required respiratory maneuvers, and forced expiratory volume in the first second (FEV₁) has poor sensitivity in evaluating peripheral airway disease [4]. Similarly, chest radiographs are also not sensitive for detecting early disease [5]. High resolution computed tomography (HRCT) is well-established and is the current “gold standard” method for monitoring lung anatomical changes in patients with CF [6]. To facilitate evaluation and comparison of quantitative data, investigators have developed quantitative scoring systems based on CT findings. The Bhalla scoring system [7], widely used particularly in pediatric and adult patients with CF, is a scoring system that has been demonstrated to correlate HRCT findings with clinical and physiological characteristics [8].

Studies have reported a good correlation between Bhalla scores and spirometry values in children [3,5]. The aim of this study was to evaluate the morphological changes due to CF lung disease in patients according to HRCT findings and to correlate the HRCT scores obtained using the Bhalla scoring system with clinical and laboratory indicators.

METHODS

Patient population

This retrospective study consisted of a review of all the medical records of children with a confirmed diagnosis of CF who were followed up at Department of Pediatric Allergy and Immunology, Cukurova University Balcali Hospital between March 2011 and January 2016. Patients who underwent chest HRCT because of persistent wheezing or collapse and who underwent PFTs within a month of HRCT were included in the study. CF was diagnosed by standard sweat electrolyte testing and chromosomal analysis in all patients [9-11]. CFTR whole-genome sequencing was performed. Clinical data, including demographic characteristics, physical examination findings, age at application, follow-up period, deep throat and sputum cultures, PFTs, and chest HRCT findings, were obtained from the medical records of the patients. Weight, height, and body mass index (BMI) were used for clinical evaluation. The weight and height of the patients were compared with the mean weight and height of healthy Turkish children of the same age and sex [12]. The patients were divided into the following two groups depending on the weight and height percentiles: (1) < 3rd percentile and (2) ≥ 3rd percentile. Deep throat or sputum cultures were evaluated for the presence of bacteria including Pseudomonas aeruginosa (PsA).

Pulmonary function tests

Spirometry was performed within 1 month of HRCT in patients aged ≥ 5 years. All patients were clinically stable at the time the tests were conducted. FEV₁, forced vital capacity (FVC), and forced expiratory flow between 25% and 75% of FVC (FEF25–75) were expressed as the percentages of predicted values [13]. The patients were divided into two groups according to their FEV₁ value: (1) normal FEV₁ (≥ 80% of predicted values) and (2) low FEV₁ (< 80% of predicted values). Spirometry was performed according to the American Thoracic Society/European Respiratory Society guidelines [14].

Radiological study

HRCT was performed with a TSX-012B scanner (Toshiba, Tokyo, Japan) using a high-resolution technique with 2-mm slice thickness, 120 kVp, and 300 mA at 10-mm intervals, extending from the lung apices to the level of costophrenic angles. All HRCTs were performed by the same radiologist who was unaware of the clinical condition of the patients. If more than one chest HRCT had been performed for a patient, only the last scan was scored.

The Bhalla scoring system is used to evaluate CT findings (Table 1) [7,15]. The scoring system proposed by Bhalla et al. [7] for CT evaluates the following: (1) severity of bronchiectasis, (2) extent of bronchiectasis, (3) peribronchial thickening, (4) extent of mucus plugs, (5) abscesses/sacculation, (6) generalities of the bronchial division involved, (7) number of bubbles, (8) emphysema, and (9) collapse/consolidation. Scores from 0 to 3 were assigned to each of the first seven categories (severity of bronchiectasis, peribronchial thickening, extent of bronchiectasis, extent of mucus plugging, sacculations, generations of bronchi involved, and number of bubbles) and from 0 to 2 for the last two categories (emphysema and collapse/consolidation). The total score has a maximum value of 25. The total score was calculated by adding individual scores for each item, and the scores ranged from 0 (normal) to 25 (severe lung structure abnormalities).

Statistical analysis

Quantitative variables were expressed as mean ± standard deviation. Frequencies were used for categorical variables. Correlation of the Bhalla score and its categories with other variables including sex, age at diagnosis, and bacteria/PsA growth in the sputum/deep throat culture was analyzed using the Kruskal–Wallis test. Spearman’s rank correlation (r) was used to assess the correlation between the Bhalla scores (and its categories) and spirometry data and BMI. The results were considered significant at a p value of < 0.05.

The Institutional Review Board of Cukurova University Hospital reviewed and approved the study (IRB date: 10.03.2017 file No. 61/49).

RESULTS

The clinical records of 80 patients were reviewed. Of these, 28 patients (nine females) who underwent chest HRCT and PFT within 1 month of the HRCT were included in the study.

The patients underwent HRCT at a median age of 6.8 years (interquartile range [IQR], 0.9 to 17). The mean age at the time of diagnosis was 2 years 4 months (0.08 to 12 years). Twenty children (71.4%) were aged < 2 years at the time of diagnosis. The mean follow-up period was 5.57 years (range, 0.4 to 15.83).

The clinical characteristics of the studied population are presented in Table 2. There was no significant correlation between the Bhalla score and sex, age group, or height percentiles. However, a significant relationship was found between the Bhalla score and weight (p = 0.036) and BMI (p = 0.032). Moreover, a significant correlation was found between the Bhalla score and BMI (r = −3.368, p = 0.050).

Cultures

For 11 patients (39.3%), deep throat or sputum cultures were done when chest HRCT was performed. PsA, detected in the cultures of seven patients (25%), was the most commonly identified bacteria. Patients with cultures positive for PsA (p = 0.004) or other bacteria (p = 0.009) showed significantly greater Bhalla scores than those whose cultures did not test positive for bacteria.

Pulmonary function tests

PFT data were available for 14 patients (50%); the others were too young to undergo PFTs. All 14 children had bronchiectasis, including four with FEV₁ > 80% of predicted values; three among them also had normal FEF25–75 values. PFT results are presented in Table 2. There were significant correlations between the Bhalla score and FEV₁, FVC, and FEF25–75 values.

Chest HRCT

The median age at the time of chest HRCT was 6.07 ± 4.66 years. The median Bhalla score was 7.5 (IQR, 1 to 24).

Severe peribronchial thickening, severe bronchiectasis, and sacculations were demonstrated in Fig. 1. Peribronchial thickening (96.4%), bronchiectasis (75%), emphysema (64.3%), mucus plugs (50%), and collapse/consolidation (32.1%) were the most common CT abnormalities in the 28 patients. Sacculations and bullous disease were present in 10.7% and 7.1% patients, respectively. Bronchiectasis was detected in HRCT of 21 patients (75%). Bronchiectasis was mild in 15 patients (53.6%), moderate in three (10.7%), and severe in three (10.7%). Bronchiectasis was found in 1 to 5 segments in seven patients (25%), in 6 to 9 segments in five (17.9%), and in > 9 segments in nine (32.1%). Three patients had sacculations secondary to bronchiectasis involving 1 to 5 segments in one patient and > 9 segments in two.

There was peribronchial thickening in 27 patients (96.4%). Of these, 21 patients (75%) had mild, three (14.3%) had moderate, and two (7.1%) had severe peribronchial thickening.

Mucus plugs were detected in 14 patients (50%), including 1 to 5 segments in five patients (17.9%), 5 to 9 segments in seven (25%), and > 9 segments in two (7.1%). Collapse/consolidation was detected in nine patients (32.1%), including subsegmental collapse in five patients and segmental collapse in four. Emphysema was identified in 18 patients, with 1 to 5 segments in nine patients (32.1%) and > 5 segments in nine (32.1%). Correlations between the Bhalla score and PFTs are shown in Table 3 and Fig. 2. Bhalla sub-scores and their relationship with the presence of PsA are shown in Table 4.

DISCUSSION

The main cause of mortality in patients with CF is lung disease. For this reason, it is critical to improve pulmonary diagnosis in patients to better predict adverse events and monitor therapies [5]. Currently, different variables, including chest radiography, PFTs, sputum cultures, and clinical examinations, are used to assess pulmonary changes in patients with CF [16]. Spirometry is a fast and noninvasive way to measure airflow obstruction in medium-to-large airways, but is less sensitive to small airway function [17,18]. Spirometry also requires significant respiratory effort and is unsuitable for children aged < 6 years [5]. Chest radiography typically show minimal and nonspecific abnormalities [3], are less sensitive than HRCT for the detection of CF lung disease [19], and are relatively insensitive during pulmonary exacerbations [20]. Chest radiography cannot accurately define bronchiectasis, especially in young patients [21]. For pediatric patients, it is essential to monitor the lungs as early as possible because morphological changes (i.e., mucus plugging and airway inflammation) occur during the first few months of life and are usually not detected via functional measures, e.g., FEV₁, even up to 4 years of age [22].

The advent of HRCT has enabled early detection of pathological changes, including mild degrees of bronchiectasis and mucoid impaction [23].

Because of the accepted superiority of CT over chest radiography in the evaluation of pulmonary morphology, many investigators have developed CT scoring systems for patients with CF [7,24-26]. In 1991, two independent publications by Nathanson et al. [27] and Bhalla et al. [7] proposed two distinct scoring systems for the evaluation of CF lung disease in children and adults. In a study comprising 16 patients aged < 12 years, Marchant et al. [21] compared these two scoring systems and showed that the Bhalla method was superior to the Nathanson method in young children with CF. Furthermore, they found a significant correlation between the final Bhalla score and FEV₁ (r = −0.65, p = 0.012) and FVC (r = −0.57, p = 0.032). In a study using the Bhalla score, Pereira et al. [28] evaluated CT findings of 23 patients with a mean age of 17.45 ± 5.75 years. A good correlation was found between the Bhalla score and FVC (r = 0.71, p < 0.001), FEV₁ (r = 0.83, p < 0.001), and FEF25–75 (r = 0.78, p < 0.001). On the other hand, Helbich et al. [16] evaluated the CT findings of 107 patients with an average age of 14.5 ± 7.3 years at four different time points. There was a weak correlation (r = 0.25, p < 0.0001) between the CT and PFT results. Consistent with these findings, the present study also identified a weak correlation between the Bhalla scores and FVC (r = −0.381, p < 0.05), FEV₁ (r = −0.315, p < 0.05), and FEF25–75 (r = −0,229, p < 0.05).

Other studies of HRCT scoring systems have demonstrated the sensitivity of HRCT in detecting CF lung disease. de Jong et al. [29] evaluated two sets of HRCT findings using five scoring systems (Castile, Brody, Helbich, Santamaria, and Bhalla). HRCTs were obtained from children with CF over a period of 2 years. Two simultaneous PFTs were also conducted. A striking finding of this study was that structural abnormalities as scored on HRCT increased significantly, independent of the scoring system, while PFT parameters remained unchanged or even improved. This finding suggests that HRCT is more sensitive than PFTs in the detection of early and progressive lung disease and may be useful in the follow-up of children with CF and as an outcome measure in studies that evaluate therapies being developed to reduce lung damage. Similar results were published in a study by Carpio et al. [5] that evaluated HRCT findings of 31 patients with CF in whom spirometry tests were performed 4 years later. The authors found that Bhalla scoring of the HRCT findings detected the progression of structural lung disease in patients with CF despite stable spirometric findings.

PsA infection is considered a risk factor for the development of bronchiectasis in children with CF [30]. All the patients in this study who were positive for PsA had bronchiectasis. Four patients (50%) had mild bronchiectasis, one (12.5%) had moderate bronchiectasis, and three (37.5%) had severe bronchiectasis. The results of this study also found that the Bhalla scores correlated with respiratory cultures. The presence of bacteria in the deep throat or sputum cultures correlated with the severity of bronchiectasis (p = 0.028) and the extent of mucus plugs (p = 0.013). Martinez-Garcia et al. [31] found that bronchiectasis is related to pathological bacterial colonization, mainly by Pseudomonas species, and that this may be a predictor of exacerbations. These results further support the notion that it may be advantageous to include HRCT in the standard follow-up of patients with CF. Correlation between Bhalla scores and PsA status would reflect clinically meaningful disease progression [32]. Robinson et al. [32] used the modified Bhalla CF HRCT scoring system and found that CF HRCT scores highly correlate with the presence of PsA.

The Cystic Fibrosis Foundation recommends that nutritional status be monitored because optimal nutritional status is associated with better clinical outcomes. Nutritional status may be assessed by weight, height, and BMI. Weight-for-age percentile at 4 years of age has been associated with improved height and growth velocity as well as improved survival and fewer pulmonary exacerbations at 18 years of age [33]. A decrease in the weight-for-height percentage is associated with an increase in mortality rate [18]. Results from this study suggest that BMI is correlated with the Bhalla score and morbidity. According to the Bhalla scores, weight percentile was significantly correlated with the severity of bronchiectasis (p = 0.010), extent of bronchiectasis (p = 0.029), presence of abscess or sacculation (p = 0.028), and collapse/consolidation (p = 0.023). Height percentile was correlated only with the presence of abscess or sacculation (p = 0.018). BMI was correlated with severity of bronchiectasis (p = 0.013), peribronchial thickening (p = 0.001), and sacculations (p = 0.00).

Although the risk of radiation-induced cancer in patients with CF undergoing annual lung CT is < 0.5% [6,34], HRCT results in significant exposure to X-rays. It must be noted that the cumulative radiation dose in multiple chest radiographies may have unknown adverse effects [35,36]. Low-dose HRCT presents a significant reduction in the dose of radiation received by the patient (72% reduction when using 50 mAs and 80% reduction when using 34 mAs) compared HRCT with the usual dose (180 mAs) [37]. Magnetic resonance imaging (MRI) offers the advantage of no ionizing radiation. Intravenous contrast-enhanced lung MRI (IV-CEMRI) with gadolinium-based agents is the most common MRI of the lung [6]. To determine if MRI is sensitive enough to detect early symptoms of CF, Wielputz et al. [38] compared structural and perfusion abnormalities in healthy people (n = 26) and infants and preschool children with CF (n = 50). Abnormal lung structure and perfusion defects were clearly visible in MRI in the CF group [37]. In addition to MRI protocol standardization, there is also a need for MRI scoring systems to detect lung damage in patients with CF. IV-CEMRI has limitations, with the primary issue being insufficient sensitivity because of low proton density in lung air spaces [6]. If protocol standardization, scoring systems, and sensitivity are improved, MRI may someday become a useful tool for measuring loss of lung function in patients with CF.

One of the limitations of this study is the small size and the study’s retrospective design. A prospective and larger study is needed to confirm these results and would indeed help in further understanding CF progression and disease management. Another limitation of the study is the relatively small number of different clinical parameters measured. For instance, the number of exacerbations and quality of life were not evaluated in this study. Future studies are needed to assess the relationships between these variables.

In conclusion, this study showed the usefulness of chest HRCT scoring system in the pulmonary evaluation of children with CF. The usefulness of methods that do not employ ionizing radiation in diagnosing and monitoring lung disease in CF patients should be compared with HRCT. If it is demonstrated that these methods perform as well or better than HRCT, radiation-free scanning methods should be utilized in place of HRCT.

KEY MESSAGE

1. High resolution computed tomography (HRCT) is well-established and is the current “gold standard” method for monitoring lung anatomical changes in patients with cystic fibrosis (CF).

2. To facilitate evaluation and comparison of quantitative data, investigators have developed quantitative scoring systems based on computed tomography findings.

3. Bhalla chest HRCT scoring system is useful for pulmonary evaluation of children with CF.

Conflict of Interest

No potential conflict of interest relevant to this article was reported.

Figure 1.

A is severe bronchiectasis and sacculations. B is peribronchial thickening, in high resolution computed tomography scan.

Figure 2.

(A) Dot graph for correlation between Bhalla score and forced expiratory volume in the first second (FEV₁). (B) Dot graph for correlation between Bhalla score and forced vital capacity (FVC).

Table 1.

Bhalla score

Category	Score
Category	0	1	2	3
Severity of bronchiectasis	Absent	Mild (light subtly greater than the adjacent vessel)	Moderate (light 2 to 3 times higher than the adjacent vessel)	Severe (light 3 times higher than the adjacent vessel)
Peribronchial thickening	Absent	Mild (thickening of the wall equal to the vessel)	Moderate (greater thickening/doubling of the vessel)	Severe (thickening 2 times greater than the vessel)
Extent of the bronchiectasis (number of lung segments)	Absent	1–5	6–9	> 9
Extent of mucus plugs (number of lung segments)	Absent	1–5	6–9	> 9
Abscesses or sacculations (number of lung segments)	Absent	1–5	6–9	> 9
Generalities of the bronchial division involved (bronchiectasis/plug)	Absent	Over the 4th generation	Over the 5th generation	Over the 6th generation and distal
Number of bubbles	Absent	Unilateral (none > 4)	Bilateral (none > 4)	> 4
Emphysema (number of lung segments)	Absent	1–5	> 5
Collapse/consolidation	Absent	Subsegmental	Segmental/ lobar

Table 2.

Characteristics of the population studied

Variable	No. (%)	Mean ± SD	Min–Max
Female sex	9 (32)
Age, yr		7.83 ± 4.79	0.9–17
Application age, yr		2.16 ± 3.61	0.08–16
Follow up period, yr		5.57 ± 4.28	0.4–15.83
Height per
≤ 3	3 (10.7)
3–10	4 (14.3)
10–25	6 (21.4)
25–50	7 (25)
50–75	4 (14.3)
75–90	2 (7.1)
90–97	2 (7.1)
Weight per
≤ 3	2 (7.1)
3–10	4 (14.3)
10–25	11 (39.3)
25–50	5 (17.9)
50–75	4 (14.3)
75–90	2 (7.1)
Spirometry
FEV₁, %	14 (50)	68 ± 15.99	49–100
FEV₁ < 80%	10 (28.5)
FVC	14 (50)	61.79 ± 12.07	44–84
FEF 25%–75%	14 (50)	78.4 ± 32.73	24–140
Culture
Pseudomonas aeruginosa	7 (25)
Other^a	4 (14.3)
Genotype
∆F508homozygos	4 (14.3)
∆F508heterozygos	4 (14.3)
Other	20 (71.4)

FEV₁, forced expiratory volume in the first second; FVC, forced vital capacity; FEF, forced expiratory flow.

^a Including; pseudomonas fluorescens (one patient), hemofilus influenza (one patient), stafilokokus aureus (one patient), streptococcus pyogenes (one patient).

Table 3.

Correlation between the Bhalla score and pulmonary function tests

Characteristic	Bhalla score
Characteristic	r	p value
FVC, %	–0.381	0.0178
FEV₁, %	–0.315	0.0272
FEF 25%–75%	–0.229	0.0431

FVC, forced vital capacity; FEV₁, forced expiratory volume in the first second; FEF, forced expiratory flow.

Table 4.

Bhalla subscore: presence of Pseudomonas aeruginosa (%)

Category	Score
Category	0	1	2	3
Severity of bronchiectasis	0	50	12.5	37.5
Peribronchial thickening	12.5	37.5	25	25
Extent of the bronchiectasis (number of lung segments)	0	25	25	50
Extent of mucus plugs (number of lung segments)	12.5	12.5	50	25
Abscesses or sacculations (number of lung segments)	62.5	12.5	0	25
Generalities of the bronchial division involved (bronchiectasis/plug)	12.5	0	0	87.5
Number of bubbles	87.5	12.5	0	0
Emphysema (number of lung segments)	25	12.5	67.5	0
Collapse/consolidation	50	25	25	0

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