Reevaluation of the impact of methicillin-resistance on outcomes in patients with <i>Staphylococcus aureus</i> bacteremia and endocarditis

Eun-Jeong Joo; Dong Ah Park; Cheol-In Kang; Doo Ryeon Chung; Jae-Hoon Song; Sang Moo Lee; Kyong Ran Peck

doi:10.3904/kjim.2017.098

Korean J Intern Med > Volume 34(6); 2019 > Article

Joo, Park, Kang, Chung, Song, Lee, and Peck: Reevaluation of the impact of methicillin-resistance on outcomes in patients with Staphylococcus aureus bacteremia and endocarditis

Original Article

Infectious diseases

Korean J Intern Med 2019;34(6):1347-1362.

Published online: January 20, 2018

DOI: https://doi.org/10.3904/kjim.2017.098

Reevaluation of the impact of methicillin-resistance on outcomes in patients with Staphylococcus aureus bacteremia and endocarditis

Eun-Jeong Joo^1,^*, Dong Ah Park^2,^*, Cheol-In Kang³, Doo Ryeon Chung³, Jae-Hoon Song³, Sang Moo Lee², Kyong Ran Peck³

¹Division of Infectious Diseases, Department of Internal Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea

²Office of Health Technology Evaluation, National Evidence-based Healthcare Collaboration Agency, Seoul, Korea

³Division of Infectious Diseases, Department of Internal Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

Correspondence to Kyong Ran Peck, M.D. Division of Infectious Diseases, Department of Internal Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Korea Tel: +82-2-3410-0329 Fax: +82-2-3410-0064 E-mail: krpeck@skku.edu

^*These authors contributed equally to this work.

Received February 13, 2017 Revised June 12, 2017 Accepted July 8, 2017

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background/Aims

Methicillin-resistant Staphylococcus aureus (MRSA) is highly prevalent in hospitals, and has recently emerged in the community. The impact of methicillin-resistance on mortality and medical costs for patients with S. aureus bacteremia (SAB) requires reevaluation.

Methods

We searched studies with SAB or endocarditis using electronic databases including Ovid-Medline, Embase-Medline, and Cochrane Library, as well as five local databases for published studies during the period January 2000 to September 2011.

Results

A total of 2,841 studies were identified, 62 of which involved 17,563 adult subjects and were selected as eligible. A significant increase in overall mortality associated with MRSA, compared to that with methicillin-susceptible S. aureus (MSSA), was evidenced by an odds ratio (OR) of 1.95 (95% confidence interval [CI], 1.73 to 2.21; p < 0.01). In 13 endocarditis studies, MRSA increased the risk of mortality, with an OR of 2.65 (95% CI, 1.46 to 4.80). When three studies, which compared mortality rates between CA-MRSA and CA-MSSA, were combined, the risk of methicillin-resistance increased 3.23-fold compared to MSSA (95% CI, 1.25 to 8.34). The length of hospital stay in the MRSA group was 10 days longer than that in the MSSA group (95% CI, 3.36 to 16.70). Of six studies that reported medical costs, two were included in the analysis, which estimated medical costs to be $9,954.58 (95% CI, 8,951.99 to 10,957.17).

Conclusions

MRSA is still associated with increased mortality, longer hospital stays and medical costs, compared with MSSA in SAB in studies published since the year 2000.

Keywords: Methicillin resistance; Staphylococcus aureus; Bacteremia; Endocarditis; Mortality

INTRODUCTION

Hospital-acquired (HA) methicillin-resistant Staphylococcus aureus (MRSA) infections are a major cause of illness and death and impose serious economic costs on patients and hospitals. The estimated number of S. aureus-related hospitalizations increased by 62% from 294,570 to 477,927, and the estimated number of MRSA-related hospitalizations more than doubled, from 127,036 to 278,203, from 1999 through 2005 in the United States [1]. Published studies on mortality for patients with S. aureus bacteremia (SAB) indicated an increased risk of mortality for patients with MRSA compared to those with methicillin-susceptible S. aureus (MSSA) bacteremia [2]. Thus bacteremia due to HA-MRSA results in increased direct medical costs and hospital stays, compared with that due to MSSA [3].

Cases of MRSA have been documented among healthy community-dwelling persons without established risk factors for MRSA acquisition, lately defined as community-associated (CA)-MRSA [4]. Community-genotype strains carrying SCCmec type IV have now emerged as a significant cause of healthcare-associated (HCA) and hospital associated (HA) infections in the USA and European countries [5-9]. Despite the epidemiologic changes in hospital MRSA strains with the encroachment of CA-MRSA into healthcare settings [9,10], whether methicillin resistance adversely affects outcomes in patients with community-associated S. aureus bacteremia is unclear [11,12]. After the year 2000, newer antimicrobial agents active against MRSA have become available to treat MRSA and are in use as alternatives for treating serious MRSA infections. The efficacy of new antibiotics in terms of reducing mortality in patients infected with S. aureus, especially MRSA, has not been verified. Furthermore, progress in high-quality clinical management has been made in the last few years as evidenced by the fact that case fatality can be reduced by hospital infection control systems [13]. These factors, including the emergence of MRSA strains with reduced vancomycin susceptibility, enhanced the controversy regarding the clinical impact of methicillin resistance on outcomes in SAB [14].

Meta-analyses by Cosgrove et al. [2] and Whitby et al. [15] comparing the mortality rate of MRSA and MSSA bacteremia found that methicillin resistance was associated with an increased mortality. In a recent meta-analysis, a significant increase in mortality associated with MRSA bacteremia was evident in the odds ratio (OR) of 1.93 (95% confidence interval [CI], 1.54 to 2.42), when 31 articles were combined with data regarding mortality associated with both MSSA and MRSA bacteremia [2]. There were also worse outcomes in studies that involve nosocomial SAB, compared to those involving a significant proportion of CA-SAB [2]. However, in the era of the emergence of CA-MRSA and the advent of newer antimicrobial agents active against MRSA, the impact of methicillin-resistance on mortality and medical costs for patients with SAB needs to be reevaluated. Therefore, we performed a systematic review and meta-analysis to investigate the effect of methicillin-resistance on mortality, length of hospital stay and medical costs of patients with SAB based on reports published after the year 2000.

METHODS

Literature search and selection of eligible studies

We searched studies of SAB or endocarditis using electronic databases including Ovid-Medline, Embase-Medline, and the Cochrane Library, as well as five local databases providing information on Korean medical research, published from January 1, 2000 to September 15, 2011. We used the search filter recommended by the Scottish Intercollegiate Guidelines Network to efficiently identify cohort studies. We also reviewed the bibliographies of relevant articles to identify additional publications. A full-text search of eight databases in English or Korean were reviewed using the terms “Staphylococcus aureus” AND “bacteremia” OR “endocarditis.” Two reviewers (D.A.P. and S.M.L.) independently evaluated titles, abstracts and citations to assess relevance for full review. We applied no language restriction in the electronic database search, which was limited to studies involving humans.

The inclusion criteria were as follows: studies (1) targeting SAB or S. aureus endocarditis (SAE); (2) comparison of outcomes of MRSA and MSSA; (3) evaluating any type of mortality, the length of hospital stay (LOS) or medical costs; and (4) involving adults 18 years older. The exclusion criteria were as follows: (1) not original research; (2) animal or pre-clinical studies; (3) not cohort studies; (4) only an abstract; (5) studies not published in Korean or English; and (6) duplicate reports. Therefore, all cohort studies in adults with SAB or endocarditis were included if they compared outcomes of MRSA to those of MSSA. Outcomes of methicillin-resistance were analyzed in terms of all-cause mortality, in-hospital mortality, SAB-related mortality, and 30-day mortality. The LOS and medical costs were also compared between the MRSA and MSSA groups. Studies involving children or neonates and those of a case-control design were excluded. We also excluded studies involving the same population during an overlapping 1-year study period.

Since this study had evaluated the published data of applicable studies, it was not required to obtain approval by the Institutional Review Board. Obtaining written informed consent was not applicable in the performance of a meta-analysis where no foreseeable harm is expected to result from the study.

Data extraction

Using a standardized form developed in advance, two independent reviewers extracted the following pre-specified data: first author, publication year, country, study period, study setting, study design, total number of study participants, the number and proportion of individuals in the MRSA and MSSA groups, age, proportion of males, cases with nosocomial- and community-acquired bacteremia, SAE, and results of predetermined outcomes during the follow-up period. We also collected the adjusted estimates of mortality in SAB and endocarditis and confounding variables considered in the statistical models of each study. Agreement was obtained after discussion between the two reviewers. We did not assess the methodological quality of included studies because most did not differ in design or in the methods used for recruiting participants.

Data synthesis and analysis

We employed a random-effects model using the method described by DerSimonian and Laird [16] to synthesize data from included studies. For the outcome data on mortality, we calculated the OR and 95% CI as summary statistics. For continuous outcomes, such as the length of hospital stay and medical costs, weighted mean differences (WMDs) and 95% CIs were calculated.

We assessed statistical heterogeneity using the Cochrane Q-test (p < 0.10) and I² statistic, with I² > 50% indicating at least moderate heterogeneity [17]. To assess the potential explanations for heterogeneity, we performed subgroup analyses using pre-specified criteria including disease characteristics (bacteremia including mixed populations and endocarditis) and the type of infection (community-acquired infections and ≥ 70% vs. < 70% nosocomial infection). We also performed sensitivity analyses using summary estimates in studies adjusted for confounding variables. First, we used a funnel plot asymmetry approach to assess publication bias qualitatively, and then we confirmed the symmetry of the funnel plot using Begg and Mazumdar’s rank correlation test (Supplementary Fig. 1) [18,19]. If publication bias was suspected, we performed the Trim and Fill method to obtain symmetry in the funnel plot and to determine the effect of hypothetical studies on the pooled estimate [20]. Statistical analysis was performed using Review Manager version 5.0 (RevMan, The Cochrane Collaboration, Oxford, UK) and Stata software version 10.0 (SE, Stata Corp., College Station, TX, USA). A p value of 0.05 was regarded as statistically significant.

RESULTS

Study populations

A total of 2,841 studies were searched from January 2000 through September 2011. Of 2,075 studies from which duplicated reports were eliminated, 92 (eight studies in Korea and 84 in other countries) were selected after the first and second literature review. A flow diagram of identification of eligible studies is shown in Fig. 1. Of these, 62 cohort studies were selected as eligible that reported any outcome regarding mortality, LOS and medical costs after review of the full-text of articles (Table 1) [21-82]. Pooled data for 17,563 patients (6,390 MRSA and 11,173 MSSA) were included in the analysis. All were cohort studies, comprising 41 retrospective, 20 prospective and one both retro- and prospective study. The characteristics of the selected studies are shown in Table 1 according to year of publication.

Mortality in patients with methicillin-resistant and methicillin-susceptible SAB and endocarditis

Of the 62 studies, 60 reported all-cause mortality including in-hospital mortality, 14- and 30-day mortality and SAB-related mortality. The clinical characteristics of all patients with MRSA and MSSA in the 62 studies are summarized in Table 1. A significant increase in all-cause mortality associated with MRSA was evident with a pooled OR of 1.95 (95% CI, 1.73 to 2.21; I² = 44%) compared to that of MSSA (Fig. 2) [21-82]. The pooled OR for 40 studies that reported in-hospital mortality was 1.90 (95% CI, 1.57 to 2.28; I² = 51%). In 13 studies that compared 30-day mortality rates in SAB, MRSA increased the odds of death 1.89-fold compared to MSSA (95% CI, 1.58 to 2.26; I² = 40%). In the 16 studies that documented SAB or infection-related mortality, generic inverse variance methods were used. The pooled OR was 2.04 (95% CI, 1 63 to 2.55; I² = 40%).

Of the 62 selected studies, 13 reported the outcomes of SAE, among which 10 involved a population with SAE [30,35,40,47,55,61,64,65,68,71], and the remaining three reported outcomes of SAE as part of SAB episodes [26,31,82]. Methicillin-resistance increased the risk of mortality by 2.65-fold in those patients (95% CI, 1.46 to 4.80; I² = 50%). There was no significant heterogeneity among the results of these studies. Further analysis primarily involving the SAE population showed a pooled OR of 3.32 (95% CI, 1.68 to 6.59).

Mortality in patients with methicillin-resistant and methicillin-susceptible SAB and endocarditis in the Korean population

In a meta-analysis of eight studies which reported all-cause mortality in SAB and endocarditis in the Korean population, methicillin-resistance was associated with increases in mortality with a pooled OR of 3.14 (95% CI, 1.48 to 6.67) (Fig. 3) [24,26,35,51,52,64,69,71]. There was significant heterogeneity among the results of these studies (I² = 76%). Of eight studies, three studies analyzed the outcomes of SAE in Korean populations [35,64,71]; in these, the mortality risk of MRSA increased 14.19-fold compared to that of MSSA (95% CI, 3.84 to 52.41).

Community- and hospital-acquired SAB

Twenty-two studies reported outcomes of CA-SAB; of these, only three studies compared mortality rates between CA-MRSA and CA-MSSA. MRSA increased the odds 3.23-fold, compared to MSSA (95% CI, 1.25 to 8.34) when the three studies were combined (Fig. 4) [31,39,74]. Forty-one studies reported the outcomes in patients of nosocomial SAB. In the 13 selected studies in which ≥ 70% of the cases of SAB were hospital-acquired, the pooled OR was 1.70 (95% CI, 1.29 to 2.25). In contrast, in 28 studies in which less than 70% were nosocomial, the OR was 1.95 (95% CI, 1.66 to 2.29).

LOS, ICU stay, and medical costs

LOS was divided into two categories for analysis—the total LOS and the length of stay after the onset of bacteremia. Eight studies reported total LOS (Table 2) [29,37,45,63,66,67,75,82]. Of them, four studies were combined for the meta-analysis of total LOS [37,63,67,75]. The average total LOS in the MRSA group was 10.03 days longer than that in the MSSA group; this difference was significant (WMD, 10.03; 95% CI, 3.36 to 16.70; I² = 83%). The result of a sensitivity analysis, excluding the heterogeneous studies, indicated that patients with MRSA bacteremia stayed 6.72 days longer (WMD, 6.72; 95% CI, 3.38 to 10.0) than those with MSSA bacteremia without heterogeneity (I² = 31%). Among six studies that reported length of stay after the onset of bacteremia, data from two studies [62,63] were included in the analysis and showed that the average stay was 5.02 days longer in the MRSA group than the MSSA group (WMD, 5.02; 95% CI, 2.66 to 7.38), with homogeneity (I² = 0%). Four studies described the length of intensive care unit (ICU) stay. Patients with MRSA bacteremia stayed in the ICU 6.46 days longer (WMD, 6.46; 95% CI, 0.87 to 12.04), with heterogeneity among combined studies (I² = 86%) than those with MSSA. Of six studies that reported medical costs (Table 3) [33,37,46,62,63,76], two were included in the analysis, and the estimated medical costs were $9,954.58 (WMD, 9,954.58; 95% CI, 8,951.99 to 10,957.17) with a statistically significant difference between groups and homogeneity between the two studies (I² = 0%) [62,63].

Publication bias

We generated contour-enhanced funnel plots to evaluate the presence of potential publication bias for the meta-analyses performed in our review (Supplementary Fig. 1). No evidence of publication bias was noted in the funnel plots and the adjusted rank correlation tests in the meta-analyses for all-cause mortality (p = 0.10), in-hospital mortality (p = 0.056), 30-day mortality (p = 0.714), or SAB-related mortality (p = 0.557).

DISCUSSION

This systematic review of 62 relevant reports published since 2000 that evaluated the outcomes of SAB and endocarditis in adults, suggested that methicillin-resistant isolates is associated with increased mortality, hospital stay and medical costs, compared with susceptible isolates. A significant increase in all-cause mortality associated with MRSA, compared to that with MSSA compromised of 17,565 patients from 62 combined studies was evident with a pooled OR of 1.95. This is consistent with the report in 2003 by Cosgrove et al. [2] which had combined 31 studies with a total of 3,962 patients of an OR of mortality associated with MRSA of 1.93, compared with MSSA. In 60 studies that reported mortality outcomes, the relative risk (RR) was estimated to be 1.59 based on the mean mortality rate of 33.1% (2,101/6,338) in the MRSA group and 18.2% (2,014/11,075) in the MSSA group. In studies of in-hospital mortality, the RR was estimated at 1.54. This is also similar to the RR of 1.42 reported by Cosgrove et al. [2]. In the analysis involving SAB-related mortality, the pooled OR was 2.04 (95% CI, 1.63 to 2.55; I² = 40%). This was compatible with the OR of 2.2 (95% CI, 1.2 to 3.8) reported by Cosgrove et al. [2]. In this analysis reevaluating the impact of methicillin-resistance on mortality in the era of the changing epidemiology and treatment of MRSA infections, a similar trend for a strong association between methicillin-resistance and a significantly increased mortality risk was identified through a review of the literature. Through a systematic review using a database published since the year 2000, two studies reported length of stay after the onset of bacteremia; patients in the MRSA group stayed 5.02 days longer than those in the MSSA group (95% CI, 2.66 to 7.38).

We also intended to evaluate the risk of mortality in endocarditis by comparing the groups with MRSA and MSSA. Interestingly, methicillin resistance increased the risk of mortality in SAE by 2.65 (95% CI, 1.46 to 4.80). Further analysis, involving primarily the SAE population, showed a pooled OR of 3.32 (95% CI, 1.68 to 6.59), which is higher than that reported by Cosgrove et al. [2] 1.79 (95% CI, 0.84 to 3.81). This is different than our expectation that mortality would be lower among patients with MRSA endocarditis as a consequence of better management with new antibiotics. Although glycopeptides were the only treatment option for MRSA infections before 2000, new treatment agents, including daptomycin and linezolid, for MRSA have been introduced since that time. Hence, the outcomes in the MRSA group were expected to be better than those in the past, especially in SAE, which is a severe form of SAB. The increased risk of mortality in the SAE group is attributable in part to the delay between data collection and publication. More than half of the study population was collected before 2000. Besides, only fifteen studies specified the treatment regimens for SAB; the mainstay of therapy for these was limited to glycopeptides. There was no study evaluating the clinical outcomes of SAB according to the treatment regimens between glycopeptides and the new anti-MRSA agents among the 62 relevant studies. Thus, the estimated risk in our analysis does not fully reflect changes in treatment of MRSA infections using new antibiotics as alternatives to glycopeptides. Further studies are required to evaluate the risk of methicillin-resistance for mortality in the SAE population under treatments with antibiotics other than glycopeptides.

Traditionally, bacteremia and endocarditis are classified as either CA or HA (nosocomial). CA-MRSA infections have emerged in the past few years as an important medical problem, especially in children without traditional risk factors for healthcare-associated MRSA. To evaluate the risk for emergence of methicillin-resistance in the community, we examined the outcomes of 22 studies reporting outcomes for CA-SAB as part of SAB; of these, three studies reported outcomes by comparing CA-MRSA and CA-MSSA. Interestingly, methicillin-resistance increased the risk of death by 3.23 (95% CI, 1.25 to 8.34). Furthermore, in nosocomial SAB, methicillin-resistance had a relatively low risk of morality in adults with ≥ 70% HA-SAB, compared to those with < 70% HASAB. These findings are opposed to previous reports in adults, which have described non-severe outcomes in CA-SAB compared to those in HA-SAB with a few notable exceptions. Given the different distribution pattern of CA- and HA-SAB, empiric antimicrobial therapy for CA-SAB could be less appropriate than for patients with HA-SAB. Since clinical practice guidelines for the treatment of MRSA often do not recommend coverage for CA-MRSA, the association between the presence of CA-MRSA and mortality in SAB suggests that patients with CA-MRSA were more likely to have received antibiotics not effective against methicillin-resistant strains [83]. Heterogeneity among study results, however, was detected in subgroup analyses; thus, further studies are required to determine the impact of methicillin-resistance on outcomes in adults with CA-SAB.

This study had several limitations. First, we included all adult subjects irrespective of disease patterns and severity of illness in this meta-analysis; this wide distribution of subject characteristics may result in heterogeneity between the combined studies. In this study, however, the heterogeneity test results were considerably lower than those in the general meta-analysis by Cosgrove et al. [2]. When we assessed the statistical heterogeneity with I² > 50% as the indication of at least moderate heterogeneity, between-study statistical heterogeneity was not found in this meta-analysis (I² statistic, 44%). Twenty-two studies were selected as high-quality in the assessment of bias risk of 62 relevant papers; with these, the sensitivity analysis showed a pooled OR of 2.12 (95% CI, 1.76 to 2.55), a significantly increased risk of mortality of methicillin- resistance in SAB. Heterogeneity in the combined studies was not identified (I² = 46%). Thus heterogeneity did not have a major impact on the results. Therefore, a wide distribution of subject characteristics between studies in this meta-analysis is not considered to have had a huge impact on the results. Second, this analysis included data in part collected before the year 2000. Given that our data were collected around 2000, the mainstay of therapy for MRSA in this analysis was confined to glycopeptides; this may not fully reflect current medical treatment, in which newer antimicrobial agents active against MRSA have become available. Further study of the effect of new antimicrobial agents on mortality of patients with SAB is required.

Despite these limitations, the present systematic review of studies published since 20 suggests that methicillin-resistance is associated with increased mortality and hospital stay compared with susceptible isolates in SAB and endocarditis. In the SAE and CA-SAB infection subgroups, methicillin-resistance was associated with increased mortality.

KEY MESSAGE

1. Methicillin-resistance is still associated with increased mortality and hospital stay, compared with susceptible isolates in Staphylococcus aureus bacteremia.

2. In comparison of outcome between community-acquired methicillin-resistant and methicillin-susceptible S. aureus bacteremia, methicillin-resistance increased the risk for mortality.

Conflict of Interest

No potential conflict of interest relevant to this article was reported.

Acknowledgments

This study was supported by a grant from the Korean Healthcare Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (A102065).

Supplementary Materials

Supplementary Figure 1.

Contour-enhanced funnel plot and Begg & Mazumdar’s rank correlation test for exploring publication bias for all-cause mortality (A), in-hospital mortality (B), 30-day mortality (C), and Staphylococcus aureus bacteremia-related mortality (D).

kjim-2017-098-suppl1.pdf

Figure 1.

Flow diagram detailing reviewed articles and exclusion. DB, database.

Figure 2.

Forest plot summary of the results of 60 studies which reported all-cause mortality. MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible S. aureus; CI, confidence interval.

Figure 3.

Forest plot summary of results of eight which reported all-cause mortality in Staphylococcus aureus bacteremia and endocarditis in the Korean population. MRSA, methicillin-resistant S. aureus; MSSA, methicillin-susceptible S. aureus; CI, confidence interval.

Figure 4.

Forest plot summary of results of three studies which reported mortality rates between community-associated (CA)-methicillin-resistant Staphylococcus aureus (MRSA) and CA-methicillin-susceptible S. aureus (MSSA). CI, confidence interval.

Table 1.

Characteristics of the studies of Staphylococcus aureus bacteremia or endocarditis included in the systematic review

No.	Author	Year	Study period	Country	Total no. of cases	Proportion of HAMRSA/CAMRSA/MSSA^a,b,c	Population	IE (% of cases)	Types of outcomes	Mortality rate, %		Crude OR (95% CI)
No.	Author	Year	Study period	Country	Total no. of cases	Proportion of HAMRSA/CAMRSA/MSSA^a,b,c	Population	IE (% of cases)	Types of outcomes	MRSA	MSSA	Crude OR (95% CI)
1	Holmes [21]	2011	2007.1–2008.11	Australia	532	22.2/15.8/62.0^b	SAB		30-Day mortality	22.1	14.2	1.72 (1.09–2.71)
1	Holmes [21]	2011	2007.1–2008.11	New Zealand	532	22.2/15.8/62.0^b	SAB		30-Day mortality	22.1	14.2	1.72 (1.09–2.71)
2	Kao [22]	2011	2004.1–2004.12	Taiwan	137	42.3/5.8/51.8^a	SAB		Hospital mortality	43.9	21.1	2.93 (1.38–6.19)
3	Lubart [23]	2011	2004.1–2005.12	Israel	68	66.2/33.8^c	SAB		14-Day mortality	40.0	13.0	4.44 (1.15–17.18)
4	Park [24]	2011	2003.1–2008.12	Korea	266	46.6/7.9/45.5^a	SAB		30-Day mortality	21.4	28.9	0.67 (0.38–1.17)
5	Big [25]	2010	2004.1–2008.7	USA	76	60.5/39.5^c	SAB		Hospital mortality	34.8	20.0	2.13 (0.72–6.29)
6	Kang [26]	2010	2004.9–2006.8	Korea	709	46.4/53.6^c	SAB/SAE	31 (4.4)	30-Day mortality	33.1	17.1	2.40 (1.69–3.41)
7	Khan [27]	2010	2007.7–2008.6	Qatar	53	13.2/86.8^c	SAB		Hospital mortality	28.6	28.3	1.02 (0.17–5.91)
8	Kim [28]	2010	2006.7–2008.12	Canada	684	27.9/72.1^c	SAB		30-Day mortality	25.1	15.4	1.84 (1.22–2.77)
9	Ponce-de-Le-on [29]	2010	2003.1–2007.12	Mexico	172	45.9/0/54.1^b	SAB		30-Day mortality, hos- pital mortality, 7-day mortality, LOS	21.5	21.5	1.0 (0.48–2.08)
10	Takayama [30]	2010	1990.1–2006.12	Japan	33	30.3/0/69.7^b	SAE	33 (100)	Hospital mortality	70.0	34.8	4.38 (0.88–21.71)
11	Wehrhahn [31]	2010	2-Year period	Australia	81	0/22.2/77.8^b	SAB/SAE	15 (18.5)	30-Day mortality	22.2	11.1	2.29 (0.59–8.91)
12	Ammerlaan [32]	2009	2007.1–2007.12	Europe	334	23.1/76.9^c	SAB		30-Day mortality	26.0	23.3	1.15 (0.64–2.07)
13	Ben-David [33]	2009	2000.1–2003.8	Israel	182	52.2/0/47.8^b	SAB		Hospital mortality, LOS, total medical cost	25.3	18.4	1.5 (0.74–3.06)
14	Khatib [34]	2009	2002.1–2003.6	USA	78	78.2/21.8^c	SAB		Hospital mortality	34.4	5.9	8.4 (1.04–67.79)
15	Kim [35]	2009	1995.1.–2006.12	Korea	73	16.4/26.0/57.5^b	SAE	73 (100)	Hospital mortality,	35.5	2.4	22.55 (2.72–187.07)
16	Rieg [36]	2009	2002–2007	Germany	521	12.9/0/87.1^a	SAB		Hospital mortality	41.8	18.7	3.12 (1.82–5.35)
17	Rubio-Terres [37]	2009	2005.1–2005.12	Spain	366	26.8/6.3/66.9^b	SAB		Hospital mortality, LOS (in wards), ICU stay, cost per episode bacteremia	39.7	25.3	1.94 (1.22–3.09)
18	Turnidge [38]	2009	2007.6–2008.5	Australia	1865	24.1/75.9^c	SAB		30-Day mortality	30.0	17.7	2.0 (1.57–2.55)
18	Turnidge [38]	2009	2007.6–2008.5	New Zealand	1865	24.1/75.9^c	SAB		30-Day mortality	30.0	17.7	2.0 (1.57–2.55)
19	Allard [39]	2008	1991–2005	Canada	815	8.3/0.1/91.5^a	SAB		30-Day mortality	33.3	23.1	1.67 (0.98–2.83)
20	Baroudi [40]	2008	1990.1–2006.1	USA	27	55.6/44.4^c	SAE	27 (100)	Hospital mortality	40.0	50.0	0.67 (0.14–3.09)
21	Libert [41]	2008	2002.1–2004.12	Belgium	140	31.4/12.9/55.7^b	SAB		Hospital mortality	54.8	35.9	1.90 (0.97–3.76)
22	Malani [42]	2008	2004–2005	USA	68	52.9/47.1^c	SAB		Hospital mortality	25.0	12.5	3.88 (1.29–11.68)
23	Bader [43]	2007	2003.1–2004.12	USA	135	23.0/31.8/45.2^b	SAB		Hospital mortality	33.8	18.0	2.32 (1.03–5.22)
24	Cagatay [44]	2007	2001.10–2002.12	Turkey	57	80.7/19.3^c	SAB		SAB-related mortality (30-day)	54.3	63.6	0.68 (0.17–2.65)
25	Das [45]	2007	2001–11–2002–12	UK	140	49.3/10.7/40.0^b	SAB		SAB-related mortality (within 10-day), LOS	33.3	16.1	2.61 (1.12–6.08)
26	Greiner [46]	2007	1999.12–2005.5	Germany	109	18.3/7.4/74.3^b	SAB		Total hospital cost	NR	NR	NR
27	Hsu [47]	2007	1995–2005	Taiwan	123	39.0/61.0^c	SAE	123 (100)	Hospital mortality	41.7	16.0	3.75 (1.61–8.71)
28	Wang [48]	2007	1990–2004	Taiwan	1148	74.1/25.9^c	SAB		30-Day mortality	49.8	27.6	2.60 (1.95–3.47)
29	Depuydt [49]	2006	1992–2002	Belgium	32	59.4/40.6^c	Bacteremic SAP		Hospital mortality	72.2	NR	NR
30	Guilarde [50]	2006	2000.1–2001.12	Brazil	111	55.0/45.0^c	SAB		SAB-related mortality	47.5	20.0	3.63 (1.54–8.53)
31	Heo [51]	2006	2000.1–2005.8	Korea	231	0/27.3/72.7^b	SAB		Hospital mortality	30.2	19.6	1.77 (0.91–3.41)
32	Kim [52]	2006	1999.1–2003.5	Korea	96	64.6/3.1/32.3^a	SAB		Hospital mortality	26.2	0.0	22.73 (1.32–391.68)
33	Lesse [53]	2006	1997.1–2003.12	USA	38	63.2/36.8^c	SAB		Hospital mortality	33.3	21.4	1.83 (0.4–8.49)
34	Marra [54]	2006	2003.12.15–2004.12.31	USA	91	46.2/53.8^c	SAB		Hospital mortality	26.2	4.1	8.69 (1.80–41.88)
35	Nori [55]	2006	1999.1–2004.2	USA	22	50.0/50.0^c	SAE	22 (100)	Hospital mortality	54.5	45.5	1.44 (0.27–7.71)
36	Perovic [56]	2006	1999.11–2002.10	South Africa	449	18.7/4.7/76.6^b	SAB		SAB-related mortality (14-day)	33.3	20.1	1.99 (1.23–3.23)
37	Shorr [57]	2006	2002–2003	USA	1540	21.2/6.2/72.6^a	SAB		Hospital mortality	23.5	16.4	1.57 (1.19–2.06)
38	Wyllie [58]	2006	1997.4–2004.3	UK	441	51.5/0/48.5^b	SAB		30-Day mortality	33.5	27.1	1.35 (0.90–2.04)
39	Cassettari [59]	2005	1999.5–1999.8	Brazil	163	58.9/0/41.1^b	SAB		Hospital mortality, SAB-related mortality (15-day)	44.8	29.9	1.91 (0.99–3.69)
40	DeRyke [60]	2005	1999.1–2004.4	USA	60	70.0/0/30.0^b	Bacteremic SAP		Hospital mortality, SAB-related mortality, infection-related LOS	54.8	55.6	0.97 (0.32–2.94)
41	Fowler [61]	2005	2000.6–2003.12	USA	424	26.7/6.7/66.7^a	SAE	424 (100)	Hospital mortality	29.8	23.3	1.39 (0.89–2.20)
41	Fowler [61]	2005	2000.6–2003.12	Multicontinent	424	26.7/6.7/66.7^a	SAE	424 (100)	Hospital mortality	29.8	23.3	1.39 (0.89–2.20)
42	Lodise [62]	2005	1999.1–2001.1	USA	353	39.9/8.2/51.8^b	SAB		SAB-related mortality, 30-day mortality, SAB-related LOS and hospital cost	30.6	15.3	2.44 (1.45–4.10)
43	Reed [63]	2005	1996.7.–2001.8	USA	143	37.8/62.2	SAB		Hospital-mortality, LOS, ICU stay, total hospital cost	14.8	9.0	1.76 (0.62–5.01)
44	Yoon [64]	2005	1986.3–2004.3	Korea	32	18.8/12.5/68.8^b	SAE	32 (100)	Hospital mortality	50.0	9.1	10.0 (1.48–67.55)
45	Chang [65]	2004	1988.1–2002.12	Taiwan	12	66.7/33.3^c	SAE	12 (100)	Hospital mortality	100	0	153.0 (2.58–9,077.05)
46	Cordova [66]	2004	1997.7–1999.6	Australia	501	7.8/3.2/89.0^a	SAB		Hospital mortality (within 16-day), 7-day mortality, LOS	27.3	16.8	1.86 (0.98–3.53)
47	Osmon [67]	2004	2001.12–2002.9	USA	265	36.2/19.6/44.2^a	SAB		Hospital mortality, LOS, ICU stay	13.5	16.2	0.81 (0.41–1.59)
48	Chang [68]	2003	1994.8–1996.3	USA	64	15.6/15.6/68.8^a	SAE	64 (100)	30-Day mortality, 14-Day mortality	50.0	22.7	3.40 (1.10–10.47)
49	Kim [69]	2003	1998.1–2002.3	Korea	29	48.3/51.7^c	SAB		SAB-related mortality	57.1	20.0	5.33 (1.02–27.76)
50	Melzer [70]	2003	1995.1–2000.12	UK	815	46.9/0/53.1^b	SAB		SAB-related mortality, overall mortality	29.6	13.6	2.66 (1.87–3.79)
51	Na [71]	2003	1990.1–2000.5	Korea	10	20.0/80.0^c	SAE	10 (100)	Hospital mortality	100	25	13.00 (0.45–377.47)
52	Blot [72]	2002	1992.1.–1998.12	Belgium	85	55.3/0/44.7^b	SAB		Hospital mortality, 15-day mortality, 30-day mortality, ICU stay	53.2	18.4	5.03 (1.85–13.69)
53	Campillo [73]	2002	1996.1–2001.3	France	83	90.4/0/9.6^b	SAB/peritonitis		Hospital mortality	60.0	75.0	0.5 (0.09–2.64)
54	Talon [74]	2002	1997.1–1998.12	France	99	11.1/19.2/69.7^b	SAB	51.7	SAB-related mortality (14-day)	43.3	20.3	3.00 (1.18–7.62)
55	Tumbarello [75]	2002	1991.1–2000.12	Italy	129	24.8/7.0/68.2^b	SAB		Hospital mortality, LOS	34.1	11.4	4.04 (1.61–10.17)
56	Cosgrove [76]	2001	1997.7–2000.6	USA	348	27.6/72.4^c	SAB		Hospital mortality, SAB-related mortality, SAB-related LOS and hospital charge	22.9	19.8	1.20 (0.68–2.12)
57	Morin [77]	2001	1998.1–1998.12	USA	192	9.9/5.2/84.9^a	SAB		Hospital mortality	13.8	10.4	1.37 (0.43–4.42)
58	Wisplinghoff [78]	2001	1995.12–1997.5	USA	82	48.8/0/51.2^b	SAB		Hospital mortality	25.0	23.8	1.07 (0.39–2.92)
59	Ibrahim [79]	2000	1997.6–1999.7	USA	94	48.9/51.1^c	SAB		Hospital mortality	37.0	25.0	1.76 (0.73–4.27)
60	Roghmann [80]	2000	1995.10–1998.1	USA	125	22.7/7.0/70.3^b	SAB		30-Day mortality	32.4	23.9	1.53 (0.66–3.57)
61	Selvey [81]	2000	1992–1997	Australia	504	37.3/0/62.7^b	SAB		Hospital mortality, SAB-related mortality	18.6	13.0	1.53 (0.94–2.51)
62	Soriano [82]	2000	1991.1–1998.12	Spain	908	19.9/4.8/75.2^b	SAB/SAE	31 (3.4)	SAB-related mortality (30-day), LOS	21.8	8.9	2.84 (1.88–4.28)

HA, hospital-acquired; MRSA, methicillin-resistant S. aurues; CA, community-acquired; MSSA, methicillin-susceptible S. aureus; IE, infective endocarditis; OR, odds ratio; CI, confidence interval; SAB, S. aureus bacteremia; SAE, S. aureus-associated infective endocarditis; LOS, length of hospital stay; ICU, intensive care unit; SAP, S. aureus-associated pneumonia.

^a Represents the proportion of cases which were epidemiologically defined according to CA- and HA-MRSA.

^b Represents the proportion of cases which were classified into community-onset and hospital-onset MRSA without definition of CA-MRSA.

^c Indicates the proportion of cases with MRSA, when onset of bacteremia was not defined according to the epidemiologic definition.

Table 2.

Length of hospital stay

No.	Author	Year	Populations	LOS, day		p value
No.	Author	Year	Populations	MRSA	MSSA	p value
1	Ponce-de-Leon [29]	2010	SAB	31 (1–585)^a	21 (0–140)^a	0.003
2	Rubio-Terres [37]	2009	SAB	24.8 (19.9–29.9)^b	22.66 (18.8–26.5)^b	NR
3	Das [45]	2007	SAB	14^c	8^c	0.004
4	Reed [63]	2005	SAB	16.6 ± 12.7^d	9.3 ± 8.5^d	< 0.0001
5	Cordova [66]	2004	SAB	16 (6–25, 1–211)^e	14 (7–30, 1–273)^e	NR
6	Osmon [67]	2004	SAB	22.1 ± 24.9^d	13.2 ± 13.5^d	0.001
7	Tumbarello [75]	2002	SAB	49 ± 27^d	24 ± 16^d	< 0.001
8	Soriano [82]	2000	SAB	18^f	8^f	< 0.00001

LOS, length of hospital stay; MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible S. aureus; SAB, S. aureus bacteremia; NR, not recorded.

^a Mean (range).

^b Mean (95% confidence interval).

^c Median.

^d Mean ± SD.

^e Median (interquartile range, range),

^f Mean.

Table 3.

Medical costs

No	Author	Year	Medical costs		p value
No	Author	Year	MRSA	MSSA	p value
1	Rubio-Terres [37]	2009	€11,044.59/episode^a	€9839.25/episode^a	-
2	Ben-David [33]	2009	ICU origin: $113,852 (48,961–55,001)^b	ICU origin: $42,137 (32,388–74,781)^b	ICU origin: < 0.001
2	Ben-David [33]	2009	General origin: $53,409 (32,945–84,053)^b	General origin: $35,131 (18,340–50,896)^b	General origin: 0.005
3	Greiner [46]	2006	€24,931^a	€10,573^a	< 0.05
4	Lodise [62]	2005	$21,577 (17,061–27,290)^c	$11,668 (9,550–14,223)^c	0.001
5	Reed [63]	2005	$28,297 ± 23,619^d	$16,066 ± 16,337^d	< 0.0001
6	Cosgrove [76]	2001	$26,424 (14,006–50,484)^b	$19,212 (9,999–36,548)^b	0.008

MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible S. aureus; ICU, intensive care unit.

^a Mean.

^b Median (interquartile range).

^c Mean (95% confidence interval).

^d Mean ± SD.

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