The Relationship between the Serum Intercellular Adhesion Molecule-1 Level and the Prognosis of the Disease in Lung Cancer

Hun Sub Shin; Chan Hee Jung; Hyun Duk Park; Seung Sei Lee

doi:10.3904/kjim.2004.19.1.48

Korean J Intern Med > Volume 19(1); 2004 > Article

Shin, Jung, Park, and Lee: The Relationship between the Serum Intercellular Adhesion Molecule-1 Level and the Prognosis of the Disease in Lung Cancer

Original Article

The Korean Journal of Internal Medicine 2004;19(1):48-52.

DOI: https://doi.org/10.3904/kjim.2004.19.1.48

The Relationship between the Serum Intercellular Adhesion Molecule-1 Level and the Prognosis of the Disease in Lung Cancer

Hun Sub Shin, M.D., Chan Hee Jung, M.D., Hyun Duk Park, M.D., Seung Sei Lee, M.D.

Department of Internal Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea

Correspondence to: Seung Sei Lee, M.D., Department of Internal Medicine, Kangbuk Samsung Hospital, 108, Pyeong-dong, Jongno-gu, Seoul, 110-746, Korea Tel: 82-2-2001-2084, Fax: 82-2-2001-2090, E-mail: lss987@samsung.co.kr

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background :

Adhesion molecules are related to cell-to-cell interaction and inflammatory interaction. In addition, adhesive interactions between tumor cells and adjacent cells and/or extracellular matrix play important roles in the complex process of tumor growth and development. Among these adhesion molecules, expression of intercellular adhesion molecule-1 (ICAM-1) has been identified in colon cancer, bladder cancer, lung cancer, melanoma, pancreatic cancer and hepatocellular carcinoma. In the current study, we analyzed serum ICAM-1 concentrations to investigate the relationship between the serum ICAM-1 level and prognosis in patients with lung cancer

Methods :

Serum ICAM-1 was measured in 84 patients with lung cancer according to the pathologic type and clinical stage using the ICAM-1 ELISA kit. The Kaplan-Meier method was used to analyse survival time.

Results :

There was no difference in serum ICAM-1 concentration among the different stages of lung cancer. Furthermore, there was no difference observed between histologic tumor type with regard to serum ICAM-1 concentration. Although the difference was not significant, the overall survival times of patients with a low serum ICAM-1 concentration (<306 ng/mL) was longer than that of patients with a high concentration (≥306 ng/mL) in non-small cell lung cancer patients.

Conclusion :

These results suggest that high levels of serum ICAM-1 reflect poor prognosis for patients with non-small cell lung cancer.

Keywords: Lung cancer; ICAM-1; Prognosis

INTRODUCTION

Adhesion molecules are related to cell-to-cell interaction and inflammatory interaction¹⁾. In addition, adhesive interactions between tumor cells and adjacent cells and/or extracellular matrix are known to be important in tumor growth²⁾.

One of these adhesion molecules is the Intercellular Adhesion Molecule-1(ICAM-1), which is a 90kD type-1 trans-membrane glycoprotein, and is known to result in various interactions by binding with the ligands, β2 integrin LFA-1 (CD11a/CD18) and MAC-1(CD11b/CD18) expressed in lymphocytes^3,4).

ICAM-1 is expressed on hematopoietic cells such as tissue macrophages, monocytes, activated T-cells, B-cells, tonsils, and lymph nodes, as well as on nonhematopoietic cell surfaces such as vascular endothelial and smooth muscle cells, thymus epithelial cells, and fibroblasts^5,6).

Studies reported an increased expression of ICAM-1 in colon cancer⁷⁾, bladder cancer^8,9), lung cancer^10,11), melanoma¹²⁾, pancreatic cancer^13,14), and hepatocellular carcinoma¹⁵⁾. The adhesion molecules such as ICAM-1 and vascular cell adhesion molecule-1 (VCAM-1) are implicated in metastasis and poor prognosis^16,17).

Thus, we examined the relationship between serum ICAM-1 and histologic classification, TNM stage, and survival time in the lung cancer patient.

MATERIALS AND METHODS

1. SUBJECTS

The study was done with data collected from 84 admitted patients who were diagnosed with lung cancer according to bronchoscopy, fine needle aspiration biopsy, and sputum cytology at Kangbuk Samsung hospital from January 1, 2000 to December 31, 2001. The subjects included 67 men and 20 women, whose average age was 60.5±10.8 years. According to histopathologic classification, there were 66 cases of non-small cell lung cancer (NSCLC) and 18 cases of small cell lung cancer (SCLC). NSCLC cases included 26 cases of squamous cell carcinoma, 34 cases of adenocarcinoma, and 6 cases of other cancers (2 cases of large cell carcinoma, 2 cases of carcinoid and 2 cases of bronchioloalveolar carcinoma). As for the pathologic stages of NSCLC, 1 case was in stage I, 1 was stage IIA, 3 were stage IIB, 8 were stage IIIA, 25 were stage IIIB, and 28 were stage IV. Thus, 11 cases were in a stage earlier than IIIA and their average age was 59.3±6.8 years. Fifty five cases were of stage greater than IIIB and their average age was 59.8±10.9 years. In SCLC cases, 4 patients with an average age of 60.8±13.0 years were in the limited stage, and 14 patients whose average age was 63.9±12.6 were in extended stage. Fifty nine patients were smokers with an average age of 61.0±10.3 years, and 25 patients were non-smokers with an average age of 59.2±12.1 years.

2. METHODS

Blood samples were collected from the 84 patients who were diagnosed with lung cancer using bronchoscopy, fine needle aspiration biopsy and sputum cytology. The samples were kept at room temperature for more than 30 minutes and each sample was centrifuged for 10 min at 1000×g and kept at −70°C until use. The concentration of serum ICAM-1 was measured using the ELISA kit from the R&D system. The reagent, standards, samples, and controls were prepared after the serum was diluted to 1:20. After placing 100 μL of diluted conjugate into each well, 100 μL of standard, control and sample was placed into the well. Incubation was done at room temperature for 1.5 h, aspiration and washing were repeated 6 times, and 100 μL of substrate was added. After incubation was done at room temperature for 30 min, 100 μL of stop solution was added and color development was observed within 30 minutes at 450 nm using a plate reader. Then, the degree of color development was compared with that of standard solution to calculate the concentration of serum ICAM-1 in the sample.

When the concentration of serum ICAM-1 was measured in blood samples collected from 131 normal individuals using the R&D system, the average concentration was 211 ng/mL, and average±2 SD was 115–306 ng/mL. Thus, we divided the concentration of serum ICAM-1 into the high concentration group (≥306 ng/mL) and low concentration group (<306 ng/mL) and compared the prognosis of each group.

3. STATISTICAL ANALYSIS

Data was analyzed using SPSS (version 11.0) statistical package. Each value was compared using the one-way ANOVA test. Bonferroni test was used as the post hoc test. The measured results were expressed in average (±SD). The rate of survival was determined using the Kaplan-Meier method. Statistical significance was determined at p<0.05.

RESULTS

1. Serum ICAM-1 concentrations according to the stage of cancer

Serum ICAM-1 concentrations according to the stage of cancer were determined by TNM staging in NSCLC patients (n=66). No significant difference among the different stages were present; the concentration was 323.3 ng/mL in stage I, 199.1 ng/mL in stage IIA, 363.6±85.4 ng/mL in stage IIB, 308.1±87.4 ng/mL in stage IIIA, 318.5±132.0 ng/mL in stage IIIB, and 327.7±144.4 ng/mL in stage IV (p value=0.91). The serum ICAM-1 concentration was 297.7±80.5 ng/mL in stages lower than IIIA and was 333.7±153.9 ng/mL in stages greater than IIIB, showing no significant difference (p value=0.45).

Likewise, no significant difference was present in SCLC patients (n=18), where the concentration was 385.0±141.0 ng/mL in the limited stage and 310.8±149.4ng/mL in the extended stage (p value=0.39) (Table 1).

2. Serum ICAM-1 concentrations according to histological type

The serum ICAM-1 concentration was 327.7±144.4 ng/mL in NSCLC (n=66) and was 327.3±146.9 ng/mL in SCLC (n=18), showing no significant difference between the two groups (p value=0.68).

In the case of NSCLC, this concentration was 356.9±130.9 ng/mL in adenocarcinoma (n=34), 321.4±144.5 ng/mL in squamous cell carcinoma (n=26), and 189.9±157.9 ng/mL in other cancers (n=6), showing no significant difference in serum ICAM-1 concentration between adenocarcinoma and squamous cell carcinoma (p value=0.32). This concentration in other cancers including large cell carcinoma (n=2), carcinoid (n=2), and bronchioloalveolar carcinoma (n=2) did not have statistical significance due to small sample size(Table 1).

3. Comparison of serum ICAM-1 concentration between smokers and nonsmokers

The concentration of serum ICAM-1 was 322.4±127.6 ng/mL in smokers (n=59) and 340.2±179.5 ng/mL in non-smokers (n=25), showing no significant difference between the two groups (p value=0.61) (Table 1).

4. Relationship between serum ICAM-1 concentration and prognosis

As for prognosis according to serum ICAM-1 concentration in NSCLC, the overall survival time was 11.9±1.6 months in 29 cases of the high serum ICAM-1 concentration group (≥306 ng/mL) and was 17.4±7.8 months in 46 cases of the low concentration group (<306 ng/mL), showing that the overall survival time was significantly longer in the low concentration group (p value<0.05) (Figure 1). In SCLC, the overall survival time was 14.8±2.9 months in 9 cases of the high sICAM-1 concentration group (≥306 ng/mL) and was 11.4±2.4 months in 9 cases of the low concentration group (<306 ng/mL), showing no significant difference between the two groups (p value=0.12) (Figure 2.)

DISCUSSION

Most cells in the body closely come in contact with other adjacent cells, basal membrane, or extracellular matrix. Adhesion molecules are known to mediate this contact between cell-to-cell or cell-to-extracellular matrix¹⁸⁾. One of these adhesion molecules is ICAM-1, which is a 90kD type-1 trans-membrane glycoprotein. It is known that this molecule participates in inflammatory interaction and tumor progression by binding with the ligands, β2 integrin LFA-1 (CD11a/CD18) and MAC-1 (CD11b/CD18), expressed in lymphocytes^3,4).

Tsujisaki et al reported that not only tumor cells, but also, fibroblasts surrounding the cancerous lesion would strongly express ICAM-1 when tumor cells themselves produce cytokines by activating T-cells and the level of sICAM-1 is increased at this time by being extracellularly released proportional to the level of surface ICAM-1¹⁹⁾. An increased expression of sICAM-1 was reported in colon cancer⁷⁾, bladder cancer^8,9), lung cancer^10,11), melanoma¹²⁾, pancreatic cancer^{13, 14)}, and hepatocellular carcinoma¹⁵⁾. It is also known that increased expression of serum ICAM-1 is related with metastasis and poor prognosis^{16, 17)}. We examined the effectiveness of serum ICAM-1 as an indicator for disease progression and prognosis in patients with lung cancer.

Taguchi et al. reported that tumor cells and surrounding normal cells increase the expression and secretion of ICAM-1 by lung cancer cells, causing an increase in the secretion of various cytokines. Thus, the level of sICAM-1 would increase as the disease progresses. However, we could not find significant difference according to disease stages in NSCLC and SCLC²⁰⁾. In NSCLC, Schardt et al. reported that the concentration of sICAM-1 has shown greater increases in large cell carcinoma compared to other histological types²¹⁾. On the other hand, Sprenger et al. reported that no relationship exists between histologic differences and serum ICAM-1 concentration in lung cancer²²⁾. We found no significant relationship between histological classification and serum ICAM-1 concentration in this study. The level of serum ICAM-1 could be increased in patients with not only lung cancer, but also, benign lung diseases such as tuberculosis, pneumonia, acute bronchitis, chronic asthma, and chronic obstructive lung disease²²⁾ and in the presence of risk factors of atherosclerosis such as diabetes, dyslipidemia, smoking and obesity. Thus, it would not be appropriate to determine prognostic factors of lung cancer according to serum ICAM-1 level^23–25).

Various stimuli such as hypoxia or inflammatory cytokines would induce functional failure of endothelial cells and expression of adhesion molecules such as ICAM-1 and VCAM-1, which work on cells and endothelial cells²⁶⁾. Smoking, a well known risk factor of lung cancer, could increase the expression of ICAM-1 by inducing functional failure of endothelial cells, regardless of the progression of lung cancer. Thus, we believe that we could not find significant difference in serum ICAM levels between smokers and non-smokers^{27, 28)}.

Kageshita et al. reported that the serum ICAM-1 level could be used as a non-invasive marker of liver metastasis and that the level is related to disease progression in malignant melanoma¹⁷⁾. Other authors reported that survival time decreased as serum ICAM-1 level increased in patients with malignant melanoma; thus, serum ICAM-1 levels has been proposed to be a marker influencing prognosis²⁹⁾. In NSCLC patients, we found that survival time was significantly higher in the low serum ICAM-1 group. No mechanism of prognosis according to serum ICAM-1 level has been elucidated. Because increased levels of serum ICAM-1 cause increased levels of cytokines, we have hypothesized that the increased levels of serum ICMA-1 cause tissue damage, promoting tumor migration and extravasation, and eventually inducing metastasis^{17, 30)}. In SCLC patients, no significant difference was seen between patients with low levels of serum ICAM-1 and those with high levels of serum ICAM-1. However, further studies are needed in the future because the number of SCLC patients was small.

In this study, we found that serum ICAM-1 level showed no relationship to lung cancer stage and histological type, and prognosis was significantly poorer in NSCLC patients with high serum ICAM-1 concentration. Hence, additional studies are needed with more patients to determine whether serum ICAM-1 level can be used as a marker for predicting the prognosis of NSCLC patients

Figure 1.

Survival curves of the 29 patients with low sICAM-1 concentrations (<306 ng/mL) and those of the 37 patients with high concentrations (≥306 ng/mL) in non-small cell lung cancer patients.

Figure 2.

Survival curves of the 9 patients with low sICAM-1 concentrations (<306 ng/mL) and those of the 9 patients with high concentrations (≥306 ng/mL) in small cell lung cancer patients.

Table 1.

sICAM-1 concntrations in patients with lung cancer according to clinicopathological features

Variables	n	sICAM-1 concentration (ng/mL)
Sex
Male	64	331.2±128.1
Female	20	316.8±190.0
Age
<70	67	327.4±149.5
≥70	17	328.6±124.7
Smoking history
Smoker	59	322.4±127.6
Nonsmoker	25	340.2±179.5
Histologic type
Non small cell lung cancer	66	327.7±144.4
Adenocarcinoma	34	356.9±130.9
Squamous cell carcinoma	26	321.4±144.5
Others	6	189.9±157.9
Small cell lung cancer	18	327.3±146.9
Stage
Non small cell lung cancer
≤IIIA	11	297.7±80.5
≥IIIB	55	337.7±153.9
Small cell lung cancer
limited stage	4	385.0±141.0
extensive stage	14	310.8±149.4

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