Histopathologic heterogeneity of acute respiratory distress syndrome revealed by surgical lung biopsy and its clinical implications

Article information

Korean J Intern Med. 2018;33(3):532-540
Publication date (electronic) : 2017 November 1
doi : https://doi.org/10.3904/kjim.2016.346
1Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University Hospital, Seoul, Korea
2Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea
3Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul, Korea
Correspondence to Chul-Gyu Yoo, M.D. Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea Tel: +82-2-2072-3760 Fax: +82-2-2072-0630 E-mail: cgyoo@snu.ac.kr
Received 2016 October 29; Revised 2017 March 19; Accepted 2017 May 16.



Diffuse alveolar damage (DAD) is the histopathologic hallmark of acute respiratory distress syndrome (ARDS). However, there are several non-DAD conditions mimicking ARDS. The purpose of this study was to investigate the histopathologic heterogeneity of ARDS revealed by surgical lung biopsy and its clinical relevance.


We retrospectively analyzed 84 patients with ARDS who met the criteria of the Berlin definition and underwent surgical lung biopsy between January 2004 and December 2013 in three academic hospitals in Korea. We evaluated their histopathologic findings and compared the clinical outcomes. Additionally, the impact of surgical lung biopsy on therapeutic alterations was examined.


The histopathologic findings were highly heterogeneous. Of 84 patients undergoing surgical lung biopsy, DAD was observed in 31 patients (36.9%), while 53 patients (63.1%) did not have DAD. Among the non-DAD patients, diffuse interstitial lung diseases and infections were the most frequent histopathologic findings in 19 and 17 patients, respectively. Although the mortality rate was slightly higher in DAD (71.0%) than in non-DAD (62.3%), the difference was not significant. Overall, the biopsy results led to treatment alterations in 40 patients (47.6%). Patients with non-DAD were more likely to change the treatment than those with DAD (58.5% vs. 29.0%), but there were no significant improvements regarding the mortality rate.


The histopathologic findings of ARDS were highly heterogeneous and classic DAD was observed in one third of the patients who underwent surgical lung biopsy. Although therapeutic alterations were more common in patients with non-DAD-ARDS, there were no significant improvements in the mortality rate.


Acute respiratory distress syndrome (ARDS) is an acute hypoxemic respiratory failure with diffuse pulmonary infiltrates caused by lung injury due to various underlying conditions [1]. A diagnosis of ARDS is currently established based on the clinical criteria of the Berlin definition, which were proposed in 2012 [2]. Upon histopathologic examination, ARDS typically presents with diffuse alveolar damage (DAD), which is the final histopathologic feature and a reaction of the lung to various injuries [3]. However, the presence of DAD on histopathology is not mandatory for the diagnosis because ARDS is defined clinically. Several non-DAD histopathologic conditions can mimic the clinical manifestations of ARDS [4]. Therefore, ARDS can be divided into two groups based on histopathology: DAD-ARDS and non-DAD-ARDS.

Currently, there are no proven pharmacologic therapies for DAD-ARDS [5]. In contrast, specific pharmacologic and therapeutic options may be available for patients with non-DAD-ARDS (i.e., specific antimicrobial therapy for pneumonia). Therefore, after the reliable and accurate diagnosis of patients with non-DAD-ARDS, a prompt initiation of specific treatment may allow for an improved prognosis. In a clinical setting, however, differentiation between DAD-ARDS and non-DAD-ARDS is difficult. Non-invasive diagnostic methods, such as computed tomography and bronchoscopy, are not effective for this differential diagnosis. Further, there are no known biomarkers for DAD [6]. Therefore, the only approach to diagnose DAD is via histopathologic examination of the lung tissue obtained by surgical lung biopsy.

Histopathologic examination has indicated that a variety of non-DAD conditions clinically manifest as ARDS. Additionally, the frequency of non-DAD-ARDS has been reported to be substantial on surgical lung biopsy [7-14]. Postmortem studies have also demonstrated that the finding of DAD could only be confirmed in approximately half of the patients with clinical ARDS [15,16]. Recently, several studies evaluating the differences between the clinical manifestation and prognosis of DAD-ARDS and non-DAD-ARDS have provided insight into the heterogeneity of ARDS [17-19]. However, in Korea, there is a lack of data concerning the frequency of non-DAD histopathologic conditions of ARDS. Therefore, the purpose of this study was to evaluate the histopathologic heterogeneity of ARDS and its clinical implications in patients in Korea.


Study population

This was a retrospective cohort study conducted in the intensive care units (ICUs) of three academic hospitals in Korea (Seoul National University Hospital, Seoul National University Bundang Hospital, and Seoul Metropolitan Government-Seoul National University Boramae Medical Center). The Institutional Review Board of each institution approved this study prior to the data collection. We included all patients with ARDS who fulfilled the criteria of the Berlin definition and who underwent surgical lung biopsy between January 2004 and December 2013. Using the Berlin definition, ARDS was categorized as mild if the partial pressure of arterial oxygen (PaO2)/fraction of inspired oxygen (FiO2) ratio was between 201 and 300 mmHg, moderate if it was between 101 and 200 mmHg, and severe if it was 100 mmHg or less [2]. The severity of ARDS was determined by the results of the arterial blood gas analysis on the date of the lung biopsy. Patients were also classified as either immunocompromised or immunocompetent, depending on their comorbidities. Immunocompromised patients were those who previously underwent organ transplantation, or who had malignant diseases, hematologic diseases, connective tissue diseases, or human immunodeficiency virus infection.

Surgical lung biopsy

Surgical lung biopsy was considered in patients with ARDS of unknown etiology after intensive evaluation, including imaging and bronchoscopy, and the decision whether to perform a biopsy was made by the attending physicians. As patients were mechanically ventilated and unable to communicate, their surrogates provided informed consent before the surgical lung biopsy. The biopsy procedure was performed in the ICU or the operating room by experienced thoracic surgeons. Patients not eligible for transport to the operating room underwent the biopsy procedure at the bedside in the ICU. The choice of surgical method between open lung biopsy via thoracotomy and video-assisted thoracoscopic surgery (VATS), and the decision regarding which lobe to resect were discussed between the thoracic surgeons and the attending physicians. Patients deemed unable to tolerate a one-lung ventilation procedure underwent open lung biopsy. Otherwise, VATS lung biopsy was attempted.

Histopathologic examination

The lung tissue obtained by surgical lung biopsy was reviewed by the pathologists at each institution. In addition to routine hematoxylin and eosin staining, other staining methods, including Gomori methenamine silver and periodic acid-Schiff staining, specific immunohistochemistry, or polymerase chain reaction assays were performed as necessary. If infection was suspected, some of the biopsied tissue was used for microbiologic cultures.

The histopathologic results of the lung biopsy were divided into two categories: DAD and non-DAD. DAD was diagnosed if hyaline membrane formation was evident and at least one of the following was observed: edema, necrosis, proliferation, or fibrosis [3]. Non-DAD histopathologic results included diffuse interstitial lung diseases, infections, and other uncommon conditions. Infections were diagnosed if specific microorganisms were identified by histopathology.

Clinical data collection

The following information was collected: age, sex, comorbidities, dates of hospital and ICU admission, Acute Physiology and Chronic Health Evaluation II (APACHE II) scores on ICU admission, results of other diagnostic evaluations performed before biopsy, dates of the surgical lung biopsy, details of biopsy procedures and results, treatment alterations made based on biopsy results, procedure-related complications, dates of ICU and hospital discharge, and in-hospital mortality.

Treatment alterations were defined as the initiation or discontinuation of drugs such as antimicrobial agents, corticosteroids, immunosuppressants, or the introduction of a new therapeutic procedure (i.e., plasmapheresis). Empirical treatment alterations that had begun prior to the report of biopsy results were not considered as treatments prescribed as a consequence of the biopsy results. Procedure-related complications were classified as either major complications or minor complications. Mortality, clinically significant hemorrhage, and reoperation for any reason after biopsy were considered major complications. The list of minor complications, on the other hand, comprised persistent air leaks, pneumothorax requiring additional chest tube insertion, and wound infection.

Statistical analysis

Data were reported as means with standard deviations for continuous variables with normal distribution, or medians with interquartile range (IQR) for those with non-normal distribution. For categorical variables, values were reported as frequencies and proportions. Differences in categorical variables between the subgroups were analyzed using either the chi-square test or Fisher exact test. Continuous variables were analyzed by Student t test or Mann-Whitney U test. A p value less than 0.05 was considered statistically significant in a two-tailed test. Statistical analyses were performed using STATA version 14.0 (StataCorp, College Station, TX, USA).


Patient characteristics and biopsy procedure

A total of 84 patients with ARDS who underwent surgical lung biopsy between January 2004 and December 2013 were analyzed. The baseline demographic characteristics and the severity of ARDS at the time of the biopsy are shown in Table 1. Their median age was 62 years (IQR, 49 to 71) and the median PaO2/FiO2 ratio at the time of the biopsy was 123.3 mmHg (IQR, 91.2 to 165.7). Among the 84 patients, 29 patients (34.5%) had severe ARDS, 45 patients (53.6%) had moderate ARDS, and the remaining ten patients (11.9%) had mild ARDS, according to the Berlin definition [2]. Thirty-eight patients (45.2%) were classified as immunocompromised due to various conditions (Table 2). The most common etiology of immunocompromised patients was connective tissue disease requiring immunosuppressants, followed by chemotherapy for malignant diseases.

Baseline characteristics of 84 patients with ARDS who underwent surgical lung biopsy

The underlying conditions of 38 immunocompromised patients

Of the 84 patients, 43 patients (51.2%) underwent open lung biopsy via thoracotomy and 41 patients (48.8%) underwent VATS lung biopsy. Patients who underwent VATS lung biopsy had a higher median PaO2/FiO2 ratio compared to those who underwent open lung biopsy (139.3 mmHg vs. 98.5 mmHg, p = 0.001). The median duration from ICU admission to lung biopsy was 2 days, and 71 patients (84.5%) underwent the biopsy procedure within 1 week after ICU admission.

Heterogeneity of histopathologic results

Histopathologic examination revealed highly heterogeneous results (Table 3). DAD was observed in 31 patients (36.9%), while non-DAD was observed in 53 patients (63.1%). When comparing the DAD-ARDS and non-DAD-ARDS groups, no differences were found between the demographic characteristics and the severity of ARDS, as determined by the PaO2/FiO2 ratio. In patients with DAD-ARDS, the median PaO2/FiO2 ratio was 140.0 mmHg and in those with non-DAD-ARDS, the median PaO2/FiO2 ratio was 112.1 mmHg (p = 0.502). The proportion of immunocompromised patients tended to be higher among patients with non-DAD-ARDS compared to those with DAD-ARDS, but this difference was not statistically significant (50.9% vs. 35.5%, p = 0.170).

Histopathologic results of surgical lung biopsy

Among the 53 patients with non-DAD-ARDS, diffuse interstitial lung diseases and infections were most commonly diagnosed in 19 and 17 patients, respectively. Most of the 19 patients diagnosed with diffuse interstitial lung diseases, including organizing pneumonia, usual interstitial pneumonia (UIP), and nonspecific interstitial pneumonia (NSIP), did not show the typical radiological findings. Therefore, it was difficult to establish a diagnosis without histopathology. Seventeen patients were diagnosed with infectious etiologies: Pneumocystis jiroveci pneumonia (n = 8), cytomegalovirus (CMV) pneumonia (n = 4), invasive pulmonary aspergillosis (n = 3), and pulmonary tuberculosis (n = 2). Other than interstitial lung diseases and infections, some patients were diagnosed with vasculitis, primary pulmonary lymphoma, or graft versus host disease. In 10 patients, the histopathologic findings were inconclusive.

Treatment alteration based on lung biopsy results

After surgical lung biopsy, 40 patients (47.6%) had therapeutic alterations based on their histopathologic results (Table 4). In these 40 patients, the most common change in their therapeutic regimen involved the initiation or withdrawal of antimicrobial agents. In 21 patients, unnecessary empirical antimicrobial agents were discontinued and in eight patients, specific antimicrobial agents were prescribed. Patients with non-DAD-ARDS were significantly more likely to change their treatment compared to those with DAD-ARDS (58.5% vs. 29.0%, p = 0.009). Particularly, in 17 patients who showed infectious etiologies on histopathology, 13 patients (76.5%) were prescribed more optimal antimicrobial therapies. In addition, corticosteroids or other immunosuppressive agents were prescribed to seven patients diagnosed with NSIP, organizing pneumonia, or graft versus host disease. Two patients diagnosed with primary pulmonary lymphoma were initiated on chemotherapy, and another two patients diagnosed with vasculitis subsequently underwent plasmapheresis.

Treatment alterations based on the histopathologic results

Clinical outcomes

Of the 84 patients included in this study, 55 patients (65.5%) died. The mortality rate was slightly higher in patients with DAD-ARDS (22 out of 31, 71.0%) than in those with non-DAD-ARDS (33 out of 53, 62.3%); however, this difference was not statistically significant (p = 0.418). Among the patients with non-DAD-ARDS, 11 out of 19 patients (57.9%) with diffuse interstitial lung diseases and 10 out of 17 patients (58.8%) with infections died.

Although therapeutic alterations occurred more frequently in patients with non-DAD-ARDS, the mortality rate did not significantly differ between patients who changed their treatment after biopsy and those who did not (57.5% vs. 72.7%, p = 0.143). Patients who had therapeutic alterations to their course of antimicrobial agents had a mortality rate of 55.2% (16 out of 29). Of the seven patients who received corticosteroids or immunosuppressants after biopsy, four patients (57.1%) died.

Procedure-related complications

After surgical lung biopsy, procedure-related complications occurred in 18 patients (21.4%). Most of the complications were minor, such as a prolonged air leak or the development of pneumothorax. However, two patients underwent reoperation to control postoperative bleeding. There were no acute procedure-related mortalities. No differences were found in the complication rates between patients who underwent VATS lung biopsy and those who underwent open lung biopsy (19.5% vs. 23.3%, p = 0.676).


ARDS is a clinical syndrome with respiratory failure caused by non-cardiogenic pulmonary edema, which results from a variety of insults to the lung. It was first described in 1967 as a collection of 12 cases of acute respiratory failure [20]. Although there has been dramatic progress in ICU care, ARDS is still a common indication for ICU admission and it remains a major cause of ICU mortality [21]. To improve the clinical recognition of ARDS, the diagnostic criteria comprising clinical and radiological findings were developed at the consensus conference of American and European investigators in 1994 [22]. The diagnostic criteria were subsequently revised as the Berlin definition in 2012 [2].

Since its description in the 1970s, ARDS has been known to typically present with DAD upon histopathologic examination [23]. However, as the diagnostic criteria for ARDS include clinical and radiological findings rather than histopathologic findings, numerous non-DAD conditions may fulfill the diagnostic criteria for ARDS on a clinical basis. Previous postmortem studies have shown that DAD was present in only half of the patients with ARDS [15,16]. As non-DAD-ARDS conditions may be treated with distinct therapeutic strategies, there have been efforts to differentiate DAD-ARDS from non-DAD-ARDS [24,25]. However, the differential diagnosis of the two conditions necessitates histopathologic examination via surgical lung biopsy, as there are currently no known specific biomarkers for DAD.

In the present study, 84 patients with ARDS underwent surgical lung biopsy to reveal the underlying conditions of ARDS, and the non-DAD histopathologic findings were observed in 63.1% of the patients. In previous studies, the frequency of non-DAD histopathologic findings in clinically diagnosed patients with ARDS varied widely, from 40% to 70% [7-14]. Selection biases might have been inevitable as most of the studies on patients with ARDS undergoing surgical lung biopsy, including our study, were retrospective cohort studies. To illustrate, atypical patients with ARDS who lack a definite precipitating cause are more often considered for surgical lung biopsy than are typical patients with ARDS who have a definite precipitating cause. This particular selection bias may be responsible for the high frequency of non-DAD histopathologic findings. The considerable proportion of immunocompromised patients also reflects this selection bias.

Compared to the patients with classic DAD-ARDS, those with non-DAD-ARDS have been reported to exhibit more favorable oxygenation and lung mechanics and have lower mortality rates [17-19]. In our study, although the mortality rate was slightly higher in patients with DAD-ARDS (71.0%) than in those with non-DAD-ARDS (62.3%), the difference was not statistically significant. There were also no significant differences in the PaO2/FiO2 ratio between patients with DAD-ARDS and those with non-DAD-ARDS. It is widely accepted that ARDS is a heterogeneous syndrome. However, no consensus exists on how to subdivide ARDS into subphenotypes until now [26]. Further studies are needed to elucidate whether it is clinically relevant to divide ARDS into DAD-ARDS and non-DAD-ARDS based on histopathologic exam.

In this study, approximately half of the patients had therapeutic alterations based on the results of the surgical lung biopsy. This proportion was slightly lower than that reported previously. The recent meta-analyses on surgical lung biopsy in ARDS reported that therapeutic changes occurred in about 80% of patients after biopsy [27,28]. This difference may have occurred because we excluded the consideration of corticosteroid therapy as a specific therapeutic alteration for DAD. We justified this decision as the evidence regarding the efficacy of corticosteroid therapy in DAD is inconclusive yet. Among the treatment alterations, the most common alteration was antibiotic de-escalation. As infection is the most common precipitating cause of ARDS, and because the clinical findings of ARDS mimic severe infection, patients with ARDS are usually treated with multiple antimicrobial agents empirically. However, it is well-known that the excessive use of broad-spectrum antimicrobial agents is responsible for several adverse drug events and the emergence of multidrug resistant microorganisms in ICUs [29]. In this regard, surgical lung biopsy may contribute to antimicrobial stewardship. However, the efficacy of de-escalation strategies in improving individual patient outcomes remains controversial [30]. This may explain why therapeutic alterations, based on surgical lung biopsy results, failed to improve mortality rate in this study.

Although this study showed that surgical lung biopsy could reveal various non-DAD histopathologic conditions, its clinical role in the diagnosis and treatment of patients with ARDS is not definitive. First, the clinical utility of lung biopsy for the detection of atypical infectious etiologies is being challenged because of the significant improvements in microbiological diagnostic techniques. Bronchoscopy and bronchoalveolar lavage, which are less invasive procedures compared to surgical lung biopsy, are very effective in identifying the infectious etiological agent of diffuse lung infiltrates, including atypical pathogens, such as P. jiroveci, CMV, and Aspergillus [31-34]. Considering the non-negligible complication rates of the surgical lung biopsy in patients with ARDS, physicians should consider such alternative methods before proceeding to surgery [28]. Second, it is unclear whether defining underlying interstitial lung diseases is helpful in the treatment of acute exacerbations presenting as ARDS because corticosteroid is the mainstay of treatment in such setting, regardless of underlying histopathology [35,36]. Although new pharmacologic options for UIP have been recently developed, extensive studies on these drugs in critically ill patients on mechanical ventilation have not been conducted [37,38].

There are critics who consider defining the underlying histopathology of ARDS to be largely an academic exercise. Ideally, a well-designed prospective study, comparing patients with ARDS who undergo surgical lung biopsy with those who do not, is necessary for a more conclusive evaluation of the benefits of the procedure. To the best of our knowledge, no such studies have been conducted. Therefore, the recommendation of surgical lung biopsy should only be made on a caseby-case basis, and a one-size-fits-all invasive approach cannot be recommended for patients with ARDS.

The present study has several differences compared to previous studies in evaluating the use of surgical lung biopsy in patients with ARDS. For instance, the sample size was substantially larger than that of previous studies. Additionally, surgical lung biopsy was performed very early on the course of ARDS (median 2 days from ICU admission). VATS biopsy was also performed in a considerable number of patients. Furthermore, this was the first study to address the various histopathologic findings of ARDS in Korea.

However, there were also limitations to be noted. First, due to the retrospective study design, we could not fully address whether the performance of surgical lung biopsy improved the patient outcomes, such as the mortality rate, length of hospitalization, or ventilator-free days. Second, only a very small proportion of patients with ARDS underwent lung biopsy, making it difficult to generalize the findings of this study. The decision to perform lung biopsy was not made at random. This selection bias should be considered when interpreting the high frequency of non-DAD-ARDS. Third, the predisposing factors to ARDS, such as pneumonia, sepsis, or trauma could not be evaluated due to the retrospective design.

In conclusion, in Korea, surgical lung biopsy in patients with ARDS revealed highly heterogeneous histopathologic results. There was no significant difference in the mortality rates between patients with non-DAD-ARDS and those with DAD-ARDS. Therapeutic alterations were made in approximately half of the patients, and patients with non-DAD-ARDS were more likely to change their treatment. However, a change in treatment did not result in a significant improvement in the mortality rate. Therefore, although surgical lung biopsy can be an option for patients with ARDS for the identification of unexpected underlying conditions, the decision to perform this procedure should be individualized.


1. Surgical lung biopsy in patients with acute respiratory distress syndrome (ARDS) revealed significant heterogeneity in the histopathology of the condition. Overall, non-diffuse alveolar damage (DAD)-ARDS was more common than classic DAD-ARDS.

2. Patients with non-DAD-ARDS were more likely to have therapeutic alterations based on histopathologic results. However, this did not lead to a significant improvement in the mortality rate.


No potential conflict of interest relevant to this article was reported.


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Article information Continued

Table 1.

Baseline characteristics of 84 patients with ARDS who underwent surgical lung biopsy

Characteristic Value
Age, yr 62 (49–71)
Male sex 51 (60.7)
PaO2/FiO2, mmHg 123.3 (91.8–164.4)
ARDS severitya
 Mild 29 (34.5)
 Moderate 45 (53.6)
 Severe 10 (11.9)
APACHE II score 23.7 ± 9.1
Biopsy method
 Open biopsy 43 (51.2)
 VATS biopsy 41 (48.8)
Days from ICU admission to biopsy 2 (0–5)
Immune status
 Immunocompetent 46 (54.8)
 Immunocompromised 38 (45.2)

Values are presented as median (interquartile range), number (%), or mean ± SD.

ARDS, acute respiratory distress syndrome; PaO2, partial pressure of arterial oxygen; FiO2, fraction of inspired oxygen; APACHE II, Acute Physiology and Chronic Health Evaluation II; VATS, video-assisted thoracic surgery; ICU, intensive care unit.


ARDS severity was determined according to the Berlin definition.

Table 2.

The underlying conditions of 38 immunocompromised patients

Cause of immunocompromised state Frequency (%)
Connective tissue disease 13 (34.2)
 Rheumatoid arthritis 3
 Systemic lupus erythematosus 3
 Dermatomyositis 2
 Churg-Strauss syndrome 1
 Polyarteritis nodosa 1
 Microscopic polyangiitis 1
 Spondyloarthropathy 1
 Polymyalgia rheumatica 1
Malignancy 11 (28.9)
 Lymphoma 5
 Lung cancer 4
 Leukemia 2
Organ transplantation 6 (15.8)
 Kidney transplantation 5
 Liver transplantation 1
Hematologic disease 5 (13.2)
 Myelodysplastic syndrome 4
 Aplastic anemia 1
Human immunodeficiency virus infection 3 (7.9)

Table 3.

Histopathologic results of surgical lung biopsy

Histopathologic result Frequency (%)
Diffuse alveolar damage 31 (36.9)
Non-diffuse alveolar damage 53 (63.1)
 Diffuse interstitial lung disease 19 (22.7)
  Organizing pneumonia 8
  Usual interstitial pneumonia 7
  Nonspecific interstitial pneumonia 4
 Infection 17 (20.2)
  Pneumocystis jiroveci pneumonia 8
  Cytomegalovirus pneumonia 4
  Invasive aspergillosis 3
  Pulmonary tuberculosis 2
 Others 17 (20.2)
  Pulmonary vasculitis 3
  Primary pulmonary lymphoma 2
  Alveolar proteinosis 1
  Graft versus host disease 1
  Inconclusivea 10

Including histopathologic results that were not distinguished as a specific disease entity, such as chronic inflammation and fibrosis.

Table 4.

Treatment alterations based on the histopathologic results

Treatment alteration Frequency (%)
Diffuse alveolar damage 31
 Withdrawal of antimicrobial agent 9 (29.0)
 No change 22 (71.0)
Diffuse interstitial lung disease 19
 Immunosuppressant 6 (31.6)
 Withdrawal of antimicrobial agent 3 (15.8)
 No change 10 (52.6)
Infection 17
 Initiation of antimicrobial agent 8 (47.1)
 Withdrawal of antimicrobial agent 5 (29.4)
 No change 4 (23.5)
Others 17
 Withdrawal of antimicrobial agent 4 (23.5)
 Plasmapheresisa 2 (11.8)
 Chemotherapya 2 (11.8)
 Immunosuppressanta 1 (5.8)
 No change 8 (47.1)

Plasmapheresis was performed for two patients who were diagnosed with vasculitis, and two patients who were diagnosed with primary pulmonary lymphoma received chemotherapy. One patient was started on immunosuppressant therapy for graft versus host disease.