Non-fasting triglyceride levels in the Korean population with and without ischemic heart disease and cerebrovascular disease

Article information

Korean J Intern Med. 2019;34(2):353-364

Publication date (electronic) : 2017 November 15

doi : https://doi.org/10.3904/kjim.2016.285

Seo Young Lee ¹^,^*, Bo Kyung Koo ¹^,²^,^*, Min Kyong Moon ¹^,²

¹Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea

²Department of Internal Medicine, Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul, Korea

Correspondence to Min Kyong Moon, M.D. Department of Internal Medicine, Seoul Metropolitan Government Seoul National University Boramae Medical Center, 20 Boramae-ro 5-gil, Dongjak-gu, Seoul 07061, Korea Tel: +82-2-870-2226 Fax: +82-2-831-2826 E-mail: mkmoon@snu.ac.kr

These authors contributed equally to this work.

Received 2016 September 5; Revised 2017 January 25; Accepted 2017 February 19.

Abstract

Background/Aims

Associations between non-fasting triglyceride (TG) levels and a risk of ischemic heart disease (IHD) and cerebrovascular accident (CVA) have been suggested in Caucasians. We aimed to investigate whether non-fasting TG levels reflect the risk of IHD/CVA in Koreans.

Methods

We conducted an analysis of patients aged ≥ 30 years from the nationwide survey database. Fasting TG was defined as a measurement taken ≥ 12 hours since the last meal. Non-fasting TG was categorized by fasting duration of 0 to 3, 4 to 7, and 8 to 11 hours.

Results

In subjects without history of IHD/CVA, diabetes, or lipid-lowering medication, the TG level was significantly elevated for 7 hours in men compared to fasting TG levels (p = 0.011); the mean TG levels were 154.9 mg/dL (standard error [SE], 13.0), 177.0 mg/dL (SE, 12.1), 148.8 mg/dL (SE, 2.8), and 141.5 mg/dL (SE, 1.4) for 0 to 3, 4 to 7, 8 to 11, and ≥12 hours’ fasting, respectively. In women, there was no difference in TG level according to fasting duration after adjustment for confounders. In men without diabetes, the TG level from 4 to 7 hours’ fasting showed a significant difference between subjects with or without IHD/CVA even after adjustments for age, body mass index, lipid medication, exercise, and dietary factors (215.1 mg/dL vs. 177.3 mg/dL, p < 0.001).

Conclusions

In men, non-fasting TG levels from 4 to 7 hours’ fasting were significantly associated with IHD/CVA, and were superior to fasting TG levels level in the significant association with the history of IHD or CVA.

Keywords: Non-fasting triglyceride; Cardiovascular disease; Myocardial ischemia; Fasting duration; Fasting triglyceride

INTRODUCTION

Triglyceride (TG) level was reported to be associated with coronary artery disease, even after adjustment for established risk factors including low density lipoprotein cholesterol (LDL-C) in several prospective cohort studies [1-3]. Furthermore, TG-lowering treatment caused a reduction in cardiovascular disease (CVD) risk in a selected population who had hypertriglyceridemia with or without a low high density lipoprotein cholesterol (HDL-C) level [4,5].

Recently, the non-fasting TG level has been reported to be independently associated with the risk of a cardiovascular event from > 10 years of prospective cohort studies in Caucasian populations [6,7]. In addition, non-fasting TG was superior to fasting TG level for predicting the risk of CVD [6]. In fact, most individuals are in the non-fasting state except for a few hours in the morning [8]; thus, non-fasting TG level might reflect the usual exposure level of TG in our bodies, and be more closely associated with the risk of atherosclerosis. TG levels normally return to baseline fasting levels 10 hours after a fat-containing meal [8]; however, in the general population with a normal diet, TG levels returned to baseline 6 hours after the last meal in the Caucasian population [7].

TG level can differ between races. Lipoprotein lipase activity and postprandial TG level was reported to be differ according to ethnicity [9]. However, there are very limited data on postprandial TG in Asian populations. Thus, we aimed to analyze the change in lipid level including TG level according fasting duration in the general Korean population using a nationwide survey. We also investigated whether the non-fasting TG level reflects the risk of ischemic heart disease (IHD) and cerebrovascular accident (CVA).

METHODS

Study participants

The Korean National Health and Nutrition Examination Survey (KNHANES), a nationwide cross-sectional survey conducted by the Ministry of Health and Welfare of Korea from January 2007 to December 2013, was used. In KNHANES, the sampling design was used based on household registries stratified by geographical area, sex, and age groups to be representative of the Korean population.

Among 38,503 individuals aged ≥ 30 years, we excluded subjects with (1) a TG level > 1,000 mg/dL to rule out cases with hereditary hypertriglyceridemia [10], (2) a history of heavy alcohol consumption, (3) presence of thyroid disease, (4) estimating glomerular filtration rate < 30 mL/min/1.73 m² or (5) no response to the questionnaire on IHD/CVA history. A final total of 28,094 subjects aged ≥ 30 years were included in the analysis. Fasting duration of each subject was evaluated using the self-questionnaire.

The study was approved by the Institutional Review Board (IRB) of Korea Centers for Disease Control and Prevention annually and the IRB approval numbers were 2007-02CON-04-P, 2008-04EXP-01-C, 2009-01CON-03- 2C, 2010-02CON-21-C, 2011-02CON-06-C, 2012-01EXP- 01-2C, and 2013-07CON-03-4C.

Measurement of metabolic parameters and disease definitions

Blood samples were drawn from the antecubital vein, processed, immediately refrigerated at 2°C to 8°C, and sent to a central laboratory. Total cholesterol, HDL-C, LDL-C, and TG levels were measured enzymatically using serum (Hitachi Automatic Analyzer 7600, Hitachi, Tokyo, Japan). Glycated hemoglobin was measured using high performance liquid chromatography (HLC- 723G7, Tosoh, Tokyo, Japan). Fasting TG was defined as the TG level measured ≥ 12 hours since the last meal. Non-fasting TG was categorized by a fasting duration of 0 to 3, 4 to 7, or 8 to 11 hours. Blood pressure was measured using a mercury sphygmomanometer (Baumanometer, Baum, Copiague, NY, USA) to the nearest 2 mmHg. Hypertension was defined when systolic blood pressure was ≥ 140 mmHg, diastolic blood pressure was ≥ 90 mmHg or the patient was receiving anti-hypertensive medicine. Diabetes was defined in subjects with an 8-hour fasting plasma glucose level of ≥ 126 mg/dL, a random blood glucose level ≥ 200 mg/dL, HbA1c ≥ 6.5% or taking antidiabetic drugs, including insulin [11]. CVA was determined when a subject was ever told that they had a stroke by a healthcare professional, and IHD was determined when a subject was ever told that they had coronary heart disease or angina by a healthcare professional. Smoking and alcohol-drinking history, and menopausal status were evaluated using the health interview survey, and heavy drinkers were defined as those who consumed over 30 g/day alcohol in men and over 20 g/day in women [12,13].

Nutritional information was collected using 24-hour recall administered by a trained dietary interviewer in the Korea Health Industry Development Institute. The 24-hour recall was based on a 1-day diary of daily food intake and nutrients were quantified using the Database Management System (Korea Health Industry Development Institute, Seoul, Korea); and daily fat and carbohydrate intake in each individual was analyzed after adjustment for body weight (g/kg/day). Those who stated performing 30-minute moderate-intensity exercise ≥ 5 days a week, 20-minute high-intensity exercise ≥ 3 days a week, or 30-minute walking ≥ 5 days a week were considered to have attained the exercise goal.

Statistical analyses

Sampling weights were used to account for the complex sampling, and all analyses were conducted independently according to sex. Statistical analyses were conducted using SPSS version 18.0 software for Windows (SPSS Inc., Chicago, IL, USA). The results are presented as means or as prevalence with standard error (SE). Binary logistic regression and linear regression analysis for categorical and continuous variables, respectively, were used to compare the TG level according to the fasting duration in each subgroup. Complex sample regression analysis applying weights with adjustment for age, body mass index (BMI), lipid-lowering medication, exercise, and dietary factors was performed to compare the TG level according to the IHD or CVA (IHD/CVA) history. Statistical significance was defined as p < 0.05.

RESULTS

Baseline characteristics of the study population

Among the entire study population (unweighted n = 28,094), 5.1% had IHD/CVA history. Subjects with IHD/CVA were significantly older compared to those without (64.6 years [SE, 0.4] vs. 49.9 years [SE, 0.1], p < 0.001). Established coronary risk factors such as hypertension, diabetes mellitus, and lipid-lowering medications were more prevalent in subjects with IHD/CVA (Table 1). They also had a significantly higher serum fasting TG level (145.9 mg/dL [SE, 3.1] vs. 130.1 mg/dL [SE, 0.7], p < 0.001) and a significantly lower HDL-C level (44.1 mg/dL [SE, 0.5] vs. 46.6 mg/dL [SE, 0.2] in men; 48.7 mg/dL [SE, 0.6] vs. 53.4 mg/dL [SE, 0.1] in women; p < 0.001 in both sex) compared to subjects without IHD/CVA. Among women, the prevalence of menopause was 58.2% (SE, 2.2%) and 30.2% (SE, 0.5%) in subjects with and without IHD/CVA, respectively (p < 0.001).

Table 1.

Baseline characteristics of study population

TG levels according to fasting time

At first, we investigated TG levels according to fasting duration in the general population. For this analysis, we excluded the subjects with IHD/CVA for generalization to the Korean population. Considering that the limited number of participants with a fasting duration of < 12 hours might weaken the statistical significance (Fig. 1), we categorized fasting duration into 0 to 3, 4 to 7, 8 to 11 hours, and ≥ 12 hours, and compared TG levels from 0 to 3, 4 to 7, and 8 to 11 hours with those from ≥ 12 hours’ fasting. As age and BMI independently affected TG level (p < 0.001 in both), we compared the TG level according to fasting duration with adjustment for age and BMI. In men, mean TG levels were 156.0 mg/dL (SE, 10.7), 181.9 mg/dL (SE, 11.6), 152.9 mg/dL (SE, 2.7), and 146.8 mg/dL (SE, 1.4) for 0 to 3, 4 to 7, 8 to 11, and ≥ 12 hours fasting, respectively. TG levels from 4 to 7 and 8 to 11 hours’ fasting were significantly higher compared to those of ≥ 12 hours fasting (age and BMI adjusted p = 0.027 and p = 0.003, respectively), whose statistical significance was maintained even after additional adjustment for exercise, and daily carbohydrate and fat intake per body weight (p = 0.033 and p = 0.004) (Table 2).

Figure 1.

Lipid level in subjects without ischemic heart disease/cerebrovascular accident, diabetes, or lipid-lowering agent according to fasting duration. (A) In men, TG level from every fasting duration was not statistically different from TG level obtained from ≥ 12 hours’ fasting. (B) In women, TG level from 8 hours’ fasting was significantly higher than that from ≥ 12 hours’ fasting (age-adjusted p = 0.017). LDL-C from 10 and 11 hours’ fasting was significantly lower than LDL-C from ≥ 12 hours’ fasting in both genders (age-adjusted p < 0.05). HDL-C, high density lipoprotein cholesterol; LDL-C, low density lipoprotein cholesterol; TG, triglyceride. a Age-adjusted p < 0.05 compared to ≥ 12 hours' fasting.

Table 2.

Non-fasting TG level compared with ≥ 12 hours’ fasting TG level in subjects without IHD/CVA

As diabetes [14] and lipid-lowering medication [15] are strong confounders of TG level, we subsequently analyzed the TG levels according to fasting duration only with those who had no diabetes and did not take lipid- lowering medication. TG levels from 4 to 7 and 8 to 11 hours’ fasting were higher than those of ≥ 12 hours fasting with statistical significance (p = 0.001 and p = 0.004, respectively). However, after adjustment for age, BMI, exercise, and daily carbohydrate and fat intake, only TG levels from 4 to 7 hours’ fasting were significantly higher compared to fasting TG levels (p = 0.011) (Table 2). Men with diabetes also showed tendency similar trend; however, there was no statistically significant difference between TG levels from 4 to 7 hours’ fasting and fasting TG levels due to the limited number of study subjects with diabetes (Table 2). In them, TG levels from 8 to 11 hours’ fasting was significantly higher than fasting TG levels even after adjustment for age, BMI, exercise, and daily carbohydrate and fat intake (p = 0.010) (Table 2).

In women, mean TG levels were 132.9 mg/dL (SE, 6.5), 124.1 mg/dL (SE, 9.2), 114.8 mg/dL (SE, 1.9), and 117.1 mg/ dL (SE, 0.8) for 0 to 3, 4 to 7, 8 to 11, and ≥ 12 hours’ fasting, respectively. After adjustment for age and BMI, there was no significant difference in TG levels according to fasting duration (Table 2). Analysis of only non-diabetic women without any lipid-lowering medication or the stratified analysis according to menopausal status also revealed no difference in TG levels according to fasting duration (Table 2).

For analysis of the non-fasting lipid profile other than TG, we only used the study subjects without any history of IHD/CVA, diabetes, or lipid-lowering medication. The HDL-C level from 8 to 11 hours’ fasting in men and 0 to 3 hours’ fasting in women was significantly lower compared to that of ≥ 12 hours’ fasting even after adjustment for age, BMI, exercise, and daily carbohydrate and fat intake per body weight (p = 0.011 and p = 0.010, respectively) (Table 3). In the case of LDL-C, the level from 4 to 7 hours and 8 to 11 hours’ fasting was statistically lower compared to that of ≥ 12 hours fasting, even after adjusting for covariates in both sexes (Table 3).

Table 3.

Non-fasting lipid profile in subjects without any of IHD/CVA, DM, or lipid-lowering agents

Association of non-fasting TG levels with IHD/CVA

Next, we investigated whether there was a difference in non-fasting TG level according to the presence or absence of IHD/CVA history. In entire men, there was no difference in TG level between subjects with and without IHD/CVA in each fasting duration (Table 4). However, in the case of men without diabetes, TG levels from 4 to 7 hours’ fasting was significantly higher in subjects with IHD/CVA (215.1 mg/dL [SE, 23.8]) compared to levels in those without IHD/CVA (177.3 mg/dL [SE, 11.6], p = 0.003). Even after adjusting for age, BMI, lipid medication, exercise, daily carbohydrate and fat intake, hypertension, and LDL-C level, its statistical significance was maintained (p < 0.001) (Table 4). By contrast, TG level of ≥ 12 hours’ fasting was 138.7 mg/dL (SE, 5.1) and 142.3 mg/ dL (SE, 1.4) in non-diabetic men with and without IHD/ CVA, respectively; there was no statistically significant difference between them (Table 4).

Table 4.

Non-fasting TG levels according to presence or absence of IHD/CVA history

In the case of women, those with IHD/CVA had significantly higher TG levels compared to those without IHD/CVA in every fasting duration except 4 to 7 hours, irrespective of diabetes status (Table 4). The statistical difference in TG level according to IHD/CVA did not persist after adjustment for age and lipid-lowering medication (Table 4).

DISCUSSION

The current study using a nationwide survey representing the Korean population revealed that non-fasting TG level was associated with the previous history of IHD/CVA. Especially in men, the association between non-fasting TG from 4 to 7 hours’ fasting and IHD/CVA was maintained even after adjustment for confounders. Furthermore, in this group, non-fasting TG was superior to fasting TG level in the significant association with the previous history of IHD or CVA. By contrast, in the case of women, non-fasting TG or fasting TG level was not associated with IHD/CVA after adjustment for age and BMI.

Considering the usual diet pattern of the general population, most of time we are in a non-fasting state except for the time before breakfast [16]. Therefore, non-fasting TG rather than fasting TG levels might reflect the actual exposure of TG level, which could be associated with the occurrence of the diseases. As the non-fasting TG level is determined by postprandial remnant lipoprotein, which can cause atherosclerosis [17,18], non-fasting TG could be related to the risk of IHD or CVA. Furthermore, high ratio of TG to low density lipoprotein increased susceptibility to oxidation [19] and was associated with increasing factor VII and plasminogen activator inhibitor levels [20], which might promote atherosclerosis and thrombosis.

In terms of TG level according to fasting duration, TG level normally return to baseline fasting levels at 12 hours in men and 4 hours in women after the last meal in our study. Women are known to have lower postprandial TG than men because of the higher clearance capacity caused by increased lipoprotein lipase activity [21]. The metabolic clearance rate of fatty acids was higher in women compared to men [22]. Even after considering the sex difference in TG level, men in our study showed a relatively longer duration of postprandial TG elevation compared to the Caucasian population [7]. We previously reported that fasting TG is also significantly higher in the Korean population compared to the U.S. population [23]. The reason for Korean men having a higher TG level and a relative longer duration of postprandial TG level compared to the Caucasian population is not known. Genetic polymorphisms in the APOA5 [24] and APOC3 [25] genes might explain the observed ethnic differences in TG levels, i.e., the APOA5 -1131C allele is more prevalent in Asian populations compared to Caucasian populations [24] and the presence of -1131C allele was associated with a high TG level [24]. Different dietary compositions between Asians and Caucasians might also partly explain the difference. Asian populations, including the Korean population, eat more carbohydrate compared to Caucasians [26] and also consume a relative lower proportion of protein [27], which is known to exacerbate postprandial chylomicron levels [28,29]. However, considering the sex difference in the duration of postprandial hypertriglyceridemia in our study, other factors aside from genetic factors or carbohydrate consumption rate might be existed. Further analysis is needed to confirm the difference between dietary composition and postprandial lipid levels in different ethnic groups.

In our study, the association between non-fasting TG and IHD/CVA was also different between both sexes. The significant association between non-fasting TG and IHD/CVA in a multivariate model was observed only in men, which was consistent with a previous report from Japan [30]. However, there has been conflicting data on the sex difference in the association between TG level and coronary heart disease in Asia [31] based on meta- analysis in population-based, prospective studies [32]. Considering that this method of crude analysis without adjustment showed that those with IHD/CVA had significantly higher non-fasting TG than women without IHD/CVA, and that there were a very limited number of subjects with fasting ≤ 12 hours in our study, the association between non-fasting TG level and IHD/CVA should be interpreted with caution.

The association between non-fasting TG and IHD/ CVA was prominent in non-diabetic subjects in our study. However, because there was a relative small number of study subjects with diabetes, it should be interpreted with caution. The insignificant result in diabetic subgroups might be partially explained by secondary hypertriglyceridemia as a consequence of diabetic dyslipidemia [33-36], which can reduce the variance in TG level according to IHD/CVA status.

In the case of LDL-C, its level significantly decreased following 4 to 7 and 8 to 11 hours’ fasting after adjusting variables, which was consistent with results from a Copenhagen study group [14]. The decrease in postprandial LDL-C can be explained by transient hemodilution after fluid intake with a meal [14]. This hemodilution effect can be adjusted by the albumin level, however, the lack of information on albumin level in this survey prevented us from correcting the hemodilution effect in LDL-C. The same effect was observed for HDL-C in men, showing a significant decrease level in 8 to 11 hours’ fasting, while women showed a decreased level in 0 to 3 hours’ fasting, which was a similar pattern to that of TG. In addition, considering the transient hemodilution after a meal, postprandial TG level might be underestimated in our study.

There are several limitations to this study. First, since this is a cross-sectional study, a causal relationship could not be proven. Second, the questionnaires were self-reported, and there could have recall bias. Patients with relatively mild disease such as angina or transient ischemic attack could have been unintentionally omitted. Another limitation was that the cholesterol levels were not measured repeatedly in each participant according the fasting time. Furthermore, a small number of unweighted subjects measuring TG level with a short postprandial duration weakens the statistical power of our results. Lastly, even though we considered the confounders causing secondary hypertriglyceridemia such as alcohol drinking, thyroid disease, diabetes, and chronic kidney disease, we could not exclude other possible contributors of hypertriglyceridemia such as nephrotic syndrome or medications. Furthermore, we investigated the association between TG level and IHD/CVA with adjustment for lipid-lowering medication; however, we could not differentiate the fibrate and statin. However, we used the data from a nationwide survey using a large number individuals representative of the Korean population. Although we could not show a causal relationship between non-fasting TG level and IHD/CVA risk, we replicated the significant association between non-fasting TG level and CVD risk confirmed in the previous prospective cohorts performed in the Caucasian populations [6,7]. To exclude the important confounders of hypertriglyceridemia, we adopted the excluding criteria for heavy alcohol drinking, thyroid disease, and uremia. Furthermore, to investigate the TG levels according to fasting duration, we only included the subjects without any of diabetes or lipid-lowering medication for the analysis.

In conclusion, non-fasting TG level was significantly higher for 7 postprandial hours compared to fasting TG level in men. In men, non-fasting TG levels from 4 to 7 hours’ fasting were significantly associated with IHD/CVA, and it was superior to fasting TG level in the significant association with the previous history of IHD or CVA. By contrast, in the case of women, non-fasting TG or fasting TG was not associated with IHD/CVA after adjustment for age and BMI. These data may be useful to define the appropriate timing of sampling for lipid measurements in individuals in Asia. Furthermore, our study suggests that sampling non-fasting TG might be valuable for detecting an association with atherosclerotic disease in specific subgroups.

KEY MESSAGE

1. Non-fasting triglyceride (TG) level was significantly higher compared to fasting TG level for 7 postprandial hours in men.

2. In men, non-fasting TG levels from 4 to 7 hours’ fasting were significantly associated with ischemic heart disease/cerebrovascular accident (IHD/CVA), and it was superior to fasting TG level in the significant association with the previous history of IHD or CVA.

3. Sampling non-fasting TG might be valuable for detecting an association with atherosclerotic disease in specific subgroups.

Notes

No potential conflict of interest relevant to this article was reported.

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Clinical parameter	Control	IHD/CVA	p value
Unweighted number	26,672	1,422	-
Age, yr	49.9 ± 0.1	64.6 ± 0.4	< 0.001
Male sex, %	41.2 ± 0.3	51.5 ± 1.6	< 0.001
Body mass index, kg/m²	23.78 ± 0.03	24.55 ± 0.09	< 0.001
Waist circumference, cm
Male	84.3 ± 0.1	86.4 ± 0.4	< 0.001
Female	79.1 ± 0.1	84.7 ± 0.4	< 0.001
Systolic blood pressure, mmHg	117.8 ± 0.2	127.0 ± 0.6	< 0.001
Diastolic blood pressure, mmHg	76.3 ± 0.1	76.5 ± 0.4	0.509
Fasting glucose, mg/dL	98.0 ± 0.2	107.1 ± 0.9	< 0.001
Total cholesterol, mg/dL	191.2 ± 0.3	178.9 ± 1.4	< 0.001
Fasting triglycerides, mg/dL	130.1 ± 0.7	145.9 ± 3.1	< 0.001
HDL-C, mg/dL
Male	46.6 ± 0.2	44.1 ± 0.5	< 0.001
Female	53.4 ± 0.1	48.7 ± 0.6	< 0.001
LDL-C, mg/dL	115.1 ± 0.3	104.0 ± 1.3	< 0.001
Serum creatinine, mg/dL	0.84 ± 0.00	0.94 ± 0.01	< 0.001
Aspartate aminotransferase, IU/L	21.9 ± 0.1	24.1 ± 0.4	< 0.001
Alanine aminotransferase, IU/L	21.8 ± 0.1	22.9 ± 0.5	< 0.001
Hypertension, %	27.7 ± 0.4	64.3 ± 1.6	< 0.001
Diabetes mellitus, %	10.1 ± 0.2	29.9 ± 1.5	< 0.001
Lipid medication, %	4.3 ± 0.1	23.3 ± 1.4	< 0.001
Current cigarette smoker, %	27.3 ± 0.4	28.8 ± 1.6	0.330
Regular exercise, %^a	48.1 ± 0.4	48.7 ± 1.7	0.725
Menopausal status (female)
Menopause, %	30.2 ± 0.5	58.2 ± 2.2	< 0.001
Menopause + HRT, %	8.0 ± 0.2	19.7 ± 1.7	< 0.001

Table 2.

Non-fasting TG level compared with ≥ 12 hours’ fasting TG level in subjects without IHD/CVA

	Fasting duration, hr	Male					Female
	Fasting duration, hr	Number	Mean ± SE, mg/dL	p value^a	p value^b	p value^c	Number	Mean ± SE, mg/dL	p value^a	p value^b	p value^c
Entire population	0–3	130	156.0 ± 10.7	0.098	0.044	0.052	246	132.9 ± 6.5	0.012	0.121	0.135
	4–7	138	181.9 ± 11.6	0.007	0.027	0.033	126	124.1 ± 9.2	0.333	0.994	0.974
	8–11	1,940	152.9 ± 2.7	< 0.001	0.003	0.004	2,664	114.8 ± 1.9	0.002	0.535	0.691
	≥ 12	7,257	146.8 ± 1.4	-	-	-	14,168	117.1 ± 0.8	-	-	-
No diabetes and no lipid medication	0–3	102	154.9 ± 13.0	0.375	0.234	0.246	208	127.6 ± 7.1	0.026	0.125	0.154
	4–7	121	177.0 ± 12.1	0.001	0.007	0.011	108	115.0 ± 9.4	0.268	0.731	0.731
	8–11	1,652	148.8 ± 2.8	0.004	0.064	0.077	2,318	108.5 ± 1.9	0.001	0.353	0.494
	≥ 12	6,120	141.5 ± 1.4	-	-	-	12,148	111.5 ± 0.9	-	-	-
Diabetes
No	0–3	105	154.7 ± 12.6	0.427	0.253	0.260	217	128.9 ± 7.0	0.017	0.093	0.109
	4–7	126	177.3 ± 11.6	0.001	0.008	0.012	111	117.1 ± 9.6	0.345	0.981	0.952
	8–11	1,699	149.0 ± 2.8	0.003	0.054	0.061	2,404	109.4 ± 1.9	0.001	0.389	0.525
	≥ 12	6,298	142.3 ± 1.4	-	-	-	12,776	112.3 ± 0.8	-	-	-
Yes	0–3	25	167.1 ± 15.5	0.105	0.052	0.078	29	170.9 ± 17.9	0.759	0.830	0.778
	4–7	12	240.5 ± 54.8	0.538	0.377	0.310	15	182.2 ± 21.9	0.519	0.629	0.637
	8–11	241	186.7 ± 10.0	0.013	0.007	0.010	260	168.8 ± 7.9	0.951	0.879	0.897
	≥ 12	959	179.9 ± 5.1	-	-	-	1,392	164.9 ± 3.0	-	-	-
Lipid medication
No	0–3	125	156.8 ± 11.0	0.105	0.052	0.061	232	131.2 ± 6.7	0.021	0.160	0.187
	4–7	132	181.8 ± 12.1	0.007	0.026	0.033	121	120.0 ± 9.1	0.217	0.650	0.651
	8–11	1,864	151.8 ± 2.7	0.001	0.007	0.010	2,536	113.4 ± 1.9	0.004	0.513	0.692
	≥ 12	6,962	145.6 ± 1.4	-	-	-	13,258	115.6 ± 0.8	-	-	-
Yes	0–3	5	162.7 ± 18.3	0.691	0.453	0.471	13	177.1 ± 25.9	0.168	0.253	0.250
	4–7	4	159.8 ± 22.2	0.936	0.848	0.858	3	151.7 ± 51.3	0.273	0.372	0.306
	8–11	74	189.5 ± 18.5	0.129	0.118	0.126	121	142.8 ± 8.6	0.708	0.703	0.689
	≥ 12	279	176.1 ± 7.4	-	-	-	880	141.5 ± 3.3	-	-	-
Menopause (no diabetes, no lipid medication)
No	0–3						112	109.4 ± 7.6	0.487	0.313	0.340
	4–7						70	109.0 ± 11.7	0.634	0.510	0.518
	8–11						1,499	99.9 ± 2.0	0.998	0.947	0.912
	≥ 12						7,124	100.7 ± 0.9	-	-	-
Yes + HRT	0–3						17	108.3 ± 27.9	0.050	0.097	0.102
	4–7						13	123.5 ± 16.3	0.047	0.070	0.078
	8–11						147	138.8 ± 8.5	0.190	0.642	0.656
	≥ 12						1,068	134.5 ± 3.1	-	-	-
Yes	0–3						79	149.6 ± 13.8	0.144	0.410	0.440
	4–7						25	132.6 ± 20.8	0.556	0.405	0.365
	8–11						672	127.6 ± 4.6	0.006	0.061	0.084
	≥ 12						3,956	131.7 ± 1.7	-	-	-

TG, triglyceride; IHD, ischemic heart disease; CVA, cerebrovascular accident; HRT, hormone replacement therapy.

Comparing non-fasting TG level with fasting TG level (≥ 12 hours’ fasting) without adjustment.

Comparing non-fasting TG level with fasting TG level (≥ 12 hours’ fasting) group with adjustment for age and body mass index.

Comparing non-fasting TG level with fasting TG level (≥ 12 hours’ fasting) group with adjustment for age, body mass index, exercise, and daily carbohydrate and fat intake per body weight (g/day/kg).

	Fasting duration, hr	Unweighted no.	IHD/CVA (–), mg/dL, Mean ± SE	IHD/CVA (+), mg/dL, Mean ± SE	p value^a	p value^b	p value^c	p value^d	p value^e
Male
Entire population	0–3	143	157.0 ± 10.7	164.6 ± 26.9	0.740	0.622	0.655	0.589	0.633
	4–7	145	181.9 ± 11.6	194.5 ± 27.8	0.237	0.139	0.130	0.167	0.149
	8–11	2,035	152.9 ± 2.7	142.9 ± 9.0	0.599	0.107	0.034	0.066	0.116
	≥ 12	7,832	146.8 ± 1.4	144.0 ± 5.8	0.429	0.122	0.077	0.149	0.087
No diabetes	0–3	112	154.7 ± 12.6	141.2 ± 28.8	0.804	0.654	0.652	0.289	0.400
	4–7	131	177.3 ± 11.6	215.1 ± 23.8	0.003	< 0.001	< 0.001	< 0.001	< 0.001
	8–11	1,764	149.0 ± 2.8	147.7 ± 11.6	0.887	0.566	0.386	0.341	0.306
	≥ 12	6,702	142.3 ± 1.4	138.7 ± 5.1	0.795	0.342	0.291	0.541	0.414
Female
Entire population	0–3	258	132.9 ± 6.5	161.3 ± 13.5	< 0.001	0.449	0.657	0.703	0.576
	4–7	130	124.1 ± 9.2	152.2 ± 27.6	0.305	0.755	0.774	0.901	0.429
	8–11	2,759	114.8 ± 1.9	143.8 ± 11.2	0.005	0.735	0.549	0.228	0.161
	≥ 12	14,788	117.1 ± 0.8	147.7 ± 4.1	< 0.001	0.303	0.309	0.365	0.378
No diabetes	0–3	225	128.9 ± 7.0	165.6 ± 17.0	0.001	0.478	0.628	0.705	0.538
	4–7	113	117.1 ± 9.6	109.3 ± 40.7	0.871	0.604	0.600	0.900	0.394
	8–11	2,478	109.4 ± 1.9	137.2 ± 13.4	0.039	0.984	0.803	0.413	0.348
	≥ 12	13,210	112.3 ± 0.8	143.0 ± 4.7	< 0.001	0.188	0.159	0.211	0.175

Sex	Fasting duration, hr	Number	Total cholesterol, mg/dL			TG, mg/dL^c			HDL-C, mg/dL^c			LDL-C, mg/dL
Sex	Fasting duration, hr	Number	Mean ± SE	p value^a	p value^b	Mean ± SE	p value^a	p value^b	Mean ± SE	p value^a	p value^b	Mean ± SE	p value^a	p value^b
Male	0–3	102	190.3 ± 4.6	0.608	0.699	154.9 ± 13.0	0.375	0.246	48.0 ± 1.3	0.893	0.667	112.2 ± 3.8	0.267	0.339
	4–7	121	191.4 ± 3.8	0.406	0.268	177.0 ± 12.1	0.001	0.011	46.8 ± 1.2	0.164	0.308	112.2 ± 3.3	0.022	0.018
	8–11	1,652	187.9 ± 1.0	0.010	0.003	148.8 ± 2.8	0.004	0.077	46.4 ± 0.3	0.004	0.011	113.0 ± 0.9	0.001	0.001
	≥ 12	6,120	191.4 ± 0.5	-	-	141.5 ± 1.4	-	-	47.2 ± 0.2	-	-	116.8 ± 0.5	-	-
Female	0–3	208	191.9 ± 3.0	0.713	0.336	127.6 ± 7.1	0.026	0.154	51.7 ± 1.2	0.001	0.010	114.8 ± 2.9	0.624	0.261
	4–7	108	184.6 ± 3.9	0.019	0.114	115.0 ± 9.4	0.268	0.731	53.8 ± 1.3	0.049	0.120	109.4 ± 3.2	0.006	0.046
	8–11	2,318	187.6 ± 0.8	< 0.001	< 0.001	108.5 ± 1.9	0.001	0.494	54.2 ± 0.3	0.376	0.520	111.9 ± 0.7	< 0.001	< 0.001
	≥ 12	12,148	192.5 ± 0.4	-	-	111.5 ± 0.9	-	-	53.9 ± 0.2	-	-	116.6 ± 0.4	-	-